Human Her2/ErbB2 Protein (HER-HM402)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Her2, His Tag at 0.2μg/ml (100μl/well) on the plate.Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 15.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Thr23-Thr652

Catalog: HER-HM402

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Product Description Recombinant Human Her2/ErbB2 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Thr23-Thr652.
Product Category Recombinant Protein
Target Her2/ErbB2
Synonyms CD340; EGFR2; ErbB2; HER2; HER-2; herstatin; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; NGL; p185erbB2; TKR1; ERBB2; HER-2/neu; NEU; ENV; ENVW; ERVWE1; HERV-7q; HERV-W-ENV; HERV7Q; HERVW; HERVWENV
Gene/Protein ID P04626-1
Species Human
Exact Sequence Thr23-Thr652
Molecular Weight The protein has a predicted MW of 72.3 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Her2, His Tag at 0.2μg/ml (100μl/well) on the plate.Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 15.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Her2, His Tag at 0.2μg/ml (100μl/well) on the plate.Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 15.7ng/ml determined by ELISA.
The purity of Human Her2 is greater than 95% as determined by SEC-HPLC.
Human Her2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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