Product Description |
Recombinant Human Her2/ErbB2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr23-Thr652. |
Product Background |
ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Protein |
Her2/ErbB2 |
Synonyms |
CD340; EGFR2; ErbB2; HER2; HER-2; herstatin; MLN 19; MLN19; NGL; TKR1; ERBB2; NEU; ENV; ENVW; ERVWE1; HERV7Q; HERVW |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Thr23-Thr652 |
Molecular Weight |
The protein has a predicted MW of 72.3 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Her2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human Her2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). |
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Human Her2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Her2 is greater than 95% as determined by SEC-HPLC. |
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