ELISA binding curve for recombinant human Her2 (ERBB2) protein with His tag, showing interaction with anti-Her2 antibody (hFc tag). Graph plots absorbance (OD450) vs. antibody concentration, demonstrating Her2 binding activity.
Human Her2/ErbB2 Protein (HER-HM402)
Human Her2/ErbB2 Protein (HER-HM402)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Her2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Thr23-Thr652
Catalog #HER-HM402
Regular price $330.00
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Product Description

Recombinant Human Her2/ErbB2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr23-Thr652.

Product Background

ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Protein

Her2/ErbB2

Synonyms

CD340; EGFR2; ErbB2; HER2; HER-2; herstatin; MLN 19; MLN19; NGL; TKR1; ERBB2; NEU; ENV; ENVW; ERVWE1; HERV7Q; HERVW

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Thr23-Thr652

Molecular Weight

The protein has a predicted MW of 72.3 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human Her2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human Her2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test). Immobilized Human Her2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Her2 Antibody, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA (QC Test).
Human Her2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Her2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Her2 is greater than 95% as determined by SEC-HPLC. The purity of Human Her2 is greater than 95% as determined by SEC-HPLC.

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support@kactusbio.us

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