Human PSMA/FOLH1 Protein (PSM-HM110)

  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human PSMA, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Lys44-Ala750

Catalog: PSM-HM110

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Product Description Recombinant Human PSMA/FOLH1 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Lys44-Ala750.

Product Background

Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells.

Product Category

Recombinant Protein

Target

PSMA/FOLH1
Synonyms GCP2; PSMA; PSMAFGCP; FGCP; Folate hydrolase 1; FOLHNAALADase I; GCPII; GCPIImGCP; NAALAD1; NAALADase I; NAALAdase; FOLH1; GIG27; PSM; FOLH; mGCP
Accession

Species

Human

Amino Acid Range

Lys44-Ala750
Molecular Weight The protein has a predicted MW of 80.6 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human PSMA, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

N-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human PSMA, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PSMA Antibody, hFc Tag with the EC50 of 7.0ng/ml determined by ELISA (QC Test).
The purity of Human PSMA is greater than 95% as determined by SEC-HPLC.
Human PSMA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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