Name: Human TNF alpha Protein (TNF-HM40A)
Brand: KactusBio
SKU: TNF-HM40A-100UG
Price: 247.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human TNF alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 45.8ng/ml determined by ELISA (QC Test). The affinity constant of 1.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Val77-Leu233

Catalog #TNF-HM40A

Size

100UG

500UG

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Product Description	Recombinant Human TNF alpha Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val77-Leu233.
Product Background	Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Product Category	Recombinant Protein / Antibody discovery

Protein	TNF alpha
Synonyms	APC1 protein; Cachectin; DIF; TNF; TNFalpha; TNFATNF; TNFSF1A; TNFSF2; TNFA; TNFα; DIF; TNFSF2;TNF-alpha
Accession	P01375
Species	Human
Biotinylated	no
Amino Acid Range	Val77-Leu233
Molecular Weight	The protein has a predicted MW of 20.3 kDa. Due to glycosylation, the protein migrates to 21-25 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human TNF alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 45.8ng/ml determined by ELISA (QC Test). The affinity constant of 1.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human TNF alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human TNFR2, mFc Tag with an affinity constant of 1.62 nM as determined in SPR assay (Biacore T200).
	Immobilized Human TNF alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 45.8ng/ml determined by ELISA (QC Test).
	Human TNF alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human TNF alpha is greater than 95% as determined by SEC-HPLC.

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