Human TNF alpha Protein (TNF-HM40A)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TNF alpha, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 38.5ng/ml determined by ELISA. The affinity constant of 1.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Val77-Leu233

Catalog: TNF-HM40A

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Product Description Recombinant Human TNF alpha Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Val77-Leu233.
Product Category Recombinant Protein
Target TNF alpha
Synonyms APC1 protein; Cachectin; DIF; TNF; TNFalpha; TNFATNF; TNFSF1A; TNFSF2; TNFA; TNFα; DIF; TNFSF2
Gene/Protein ID P01375
Species Human
Exact Sequence Val77-Leu233
Molecular Weight The protein has a predicted MW of 20.3 kDa. Due to glycosylation, the protein migrates to 21-25 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human TNF alpha, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 38.5ng/ml determined by ELISA. The affinity constant of 1.62 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human TNF alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human TNFR2, mFc Tag with an affinity constant of 1.62 nM as determined in SPR assay (Biacore T200).
Immobilized Human TNF alpha, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 38.5ng/ml determined by ELISA.
The purity of Human TNF alpha is greater than 95% as determined by SEC-HPLC.
Human TNF alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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