Human TNF alpha Protein (TNF-HM40A)
Catalog: TNF-HM40A
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Product Description | Recombinant Human TNF alpha Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Val77-Leu233. |
Product Category | Recombinant Protein |
Target | TNF alpha |
Synonyms | APC1 protein; Cachectin; DIF; TNF; TNFalpha; TNFATNF; TNFSF1A; TNFSF2; TNFA; TNFα; DIF; TNFSF2 |
Gene/Protein ID | P01375 |
Species | Human |
Exact Sequence | Val77-Leu233 |
Molecular Weight | The protein has a predicted MW of 20.3 kDa. Due to glycosylation, the protein migrates to 21-25 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human TNF alpha, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 38.5ng/ml determined by ELISA. The affinity constant of 1.62 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag | C-His-Avi |
Express System | HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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