ELISA binding curve of human TNF alpha (His tag) with human TNFR1 (hFc tag) showing dose-dependent response.
Non-biotinylated Human TNF alpha Protein (active trimer) (TNF-HM40A)
ELISA binding curve of human TNF alpha (His tag) with human TNFR1 (hFc tag) showing dose-dependent response.
Non-biotinylated Human TNF alpha Protein (active trimer) (TNF-HM40A)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TNF alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag (Cat. TNF-HM3R2) with the EC50 of 45.8ng/ml determined by ELISA (QC Test). The affinity constant of 1.62 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Val77-Leu233
  • Other Available Proteins:
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Catalog #TNF-HM40A
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Non-biotinylated Human TNF alpha Protein (active trimer) (TNF-HM40A) Summary

Product Description

 Recombinant Human TNF alpha Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val77-Leu233.

Product Background

 Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism.Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.

Product Category

 Recombinant Protein / Antibody discovery / ADC targets

Non-biotinylated Human TNF alpha Protein (active trimer) (TNF-HM40A) Specifications/Details

Protein
 TNF alpha
Synonyms
 APC1 protein; Cachectin; DIF; TNF; TNFalpha; TNFATNF; TNFSF1A; TNFSF2; TNFA; TNFα; DIF; TNFSF2;TNF-alpha
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Val77-Leu233
Molecular Weight
 The protein has a predicted MW of 20.3 kDa. Due to glycosylation, the protein migrates to 21-25 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human TNF alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag (Cat. TNF-HM3R2) with the EC50 of 45.8ng/ml determined by ELISA (QC Test). The affinity constant of 1.62 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-His-Avi
Expression System
 HEK293
Purity
 > 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human TNF alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human TNFR2, mFc Tag (Cat. TNF-HM3R2) with an affinity constant of 1.62 nM as determined in SPR assay (Biacore T200). Human TNF alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human TNFR2, mFc Tag (Cat. TNF-HM3R2) with an affinity constant of 1.62 nM as determined in SPR assay (Biacore T200).
Immobilized Human TNF alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag (Cat. TNF-HM3R2) with the EC50 of 45.8ng/ml determined by ELISA (QC Test). Immobilized Human TNF alpha, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TNFR2, mFc Tag (Cat. TNF-HM3R2) with the EC50 of 45.8ng/ml determined by ELISA (QC Test).
Immobilized Human TNF alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human TNFR1, hFc Tag (Cat. TNF-HM2R1) with the EC50 of 3.8ng/ml determined by ELISA. Immobilized Human TNF alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human TNFR1, hFc Tag (Cat. TNF-HM2R1) with the EC50 of 3.8ng/ml determined by ELISA.
Human TNF alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Human TNF alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
The purity of Human TNF alpha is greater than 95% and the molecular weight of this protein is around 55-76 kDa as determined by SEC-MALS. The purity of Human TNF alpha is greater than 95% and the molecular weight of this protein is around 55-76 kDa as determined by SEC-MALS.
The purity of Human TNF alpha is greater than 95% as determined by SEC-HPLC. The purity of Human TNF alpha is greater than 95% as determined by SEC-HPLC.
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