Cynomolgus DLL3 Protein (DLL-CM113)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Cynomolgus DLL3, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.3 ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala27-Arg488

Catalog: DLL-CM113

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Product Description Recombinant Cynomolgus DLL3 Protein is expressed from HEK293 with His tag at the C-terminus.; It contains Ala27-Arg488.

Product Background

Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).

Product Category

Recombinant Protein

Target

DLL3
Accession

Species

Cynomolgus

Exact Sequence

Ala27-Arg488
Molecular Weight The protein has a predicted MW of 49.21 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Cynomolgus DLL3, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.3 ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1 EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in 20 mM Tris, 200 mM NaCl, 200 mM L-arginine (pH 8.5). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Cynomolgus DLL3, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 2.3 ng/ml determined by ELISA.
Cynomolgus DLL3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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