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Biotinylated Human TROP-2/TACSTD2 Protein, Ultra Low Endotoxin (TRP-HM421B-UL) Summary
Product Description |
Recombinant Biotinylated Human TROP-2/TACSTD2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His27-Thr274. |
Product Background |
Trop-2,also known as epithelial glycoprotein-1 antigen (EGP-1),is a protein that in humans is encoded by the TACSTD2 gene.Mutations of this gene result in gelatinous drop-like corneal dystrophy, an autosomal recessive disorder characterized by severe corneal amyloidosis leading to blindness. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets, ADC targets |
Biotinylated Human TROP-2/TACSTD2 Protein, Ultra Low Endotoxin (TRP-HM421B-UL) Specifications/Details
Protein |
TROP-2/TACSTD2 |
Synonyms |
EGP1; EGP-1; TROP2; GA733-1; gp50; T16; TACSTD2; TROP-2; M1S1; TACD2 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
His27-Thr274 |
Molecular Weight |
The protein has a predicted MW of 30.5 kDa. Due to glycosylation, the protein migrates to 46-55 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human TROP-2, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TROP-2 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Biotinylated Human TROP-2, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TROP-2 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). |
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Biotinylated Human TROP-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human TROP-2 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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