- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Anti-TSLP Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TSLP, His Tag with the EC50 of 1.6ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Tyr29-Gln159
Product Description |
Recombinant Biotinylated Human TSLP Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr29-Gln159. |
Product Background |
Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells. |
Product Category |
Recombinant Protein |
Protein |
TSLP |
Synonyms |
thymic stromal lymphopoietin; TSLP |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Tyr29-Gln159 |
Molecular Weight |
The protein has a predicted MW of 17.8 kDa. Due to furin cleavage site, the protein migrates to 12 kDa&25-30 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Anti-TSLP Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TSLP, His Tag with the EC50 of 1.6ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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