Biotinylated Human TSLP Protein (TSP-HM401B)
Biotinylated Human TSLP Protein (TSP-HM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-TSLP Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TSLP, His Tag with the EC50 of 1.6ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Tyr29-Gln159
Catalog #TSP-HM401B
Regular price $525.00
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Product Description

Recombinant Biotinylated Human TSLP Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr29-Gln159.

Product Background

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Product Category

Recombinant Protein

Protein

TSLP

Synonyms

thymic stromal lymphopoietin; TSLP

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Tyr29-Gln159

Molecular Weight

The protein has a predicted MW of 17.8 kDa. Due to furin cleavage site, the protein migrates to 12 kDa&25-30 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Anti-TSLP Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TSLP, His Tag with the EC50 of 1.6ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-TSLP Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TSLP, His Tag with the EC50 of 1.6ng/ml determined by ELISA. Immobilized Anti-TSLP Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TSLP, His Tag with the EC50 of 1.6ng/ml determined by ELISA.
Immobilized Human TSLPR, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TSLP, His Tag with the EC50 of 0.71ug/ml determined by ELISA (QC Test). Immobilized Human TSLPR, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TSLP, His Tag with the EC50 of 0.71ug/ml determined by ELISA (QC Test).
Biotinylated Human TSLP on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Biotinylated Human TSLP on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of-Biotinylated Human TSLP is greater than 95% as determined by SEC-HPLC. The purity of-Biotinylated Human TSLP is greater than 95% as determined by SEC-HPLC.

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