Name: Biotinylated Human TSLP Protein (TSP-HM401B)
Brand: KactusBio
SKU: TSP-HM401B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human TSLP Protein (TSP-HM401B)

Immobilized Biotinylated Human TSLP, His Avi Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.

Immobilized Anti-TSLP Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TSLP, His Tag with the EC50 of 1.6ng/ml determined by ELISA.

Biotinylated Human TSLP on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human TSLP, His Tag with an affinity constant of 0.20 nM as determined in BLI assay (Gator® Prime).

Loaded Biotinylated Human TSLP, His Tag on Anti-His-Biosensor can bind Human TSLPR, hFc Tag with an affinity constant of 1.30 nM as determined in BLI assay (Gator® Prime).

The purity of-Biotinylated Human TSLP is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human TSLP, His Avi Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
Amino Acid Range: Tyr29-Gln159
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Catalog #TSP-HM401B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human TSLP Protein (TSP-HM401B) Summary

Product Description	Recombinant Biotinylated Human TSLP Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr29-Gln159.
Product Background	Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human TSLP Protein (TSP-HM401B) Specifications/Details

Protein	TSLP
Synonyms	thymic stromal lymphopoietin; TSLP
Accession	Q969D9-1
Species	Human
Biotinylated	yes
Amino Acid Range	Tyr29-Gln159
Molecular Weight	The protein has a predicted MW of 17.8 kDa. Due to furin cleavage site, the protein migrates to 12 kDa and 25-30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human TSLP, His Avi Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human TSLP, His Avi Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
	Immobilized Anti-TSLP Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TSLP, His Tag with the EC50 of 1.6ng/ml determined by ELISA.
	Biotinylated Human TSLP on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human TSLP, His Tag with an affinity constant of 0.20 nM as determined in BLI assay (Gator® Prime).
	Loaded Biotinylated Human TSLP, His Tag on Anti-His-Biosensor can bind Human TSLPR, hFc Tag with an affinity constant of 1.30 nM as determined in BLI assay (Gator® Prime).
	The purity of-Biotinylated Human TSLP is greater than 95% as determined by SEC-HPLC.