Biotinylated Human TSLP Protein, Ultra Low Endotoxin (TSP-HM401B-UL)

Immobilized Biotinylated Human TSLP, His Avi Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.

Immobilized Anti-TSLP Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TSLP, His Tag with the EC50 of 1.6ng/ml determined by ELISA.

Biotinylated Human TSLP on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of-Biotinylated Human TSLP is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human TSLP, His Avi Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
Amino Acid Range: Tyr29-Gln159

Catalog #TSP-HM401B-UL

Price

$635²⁵

Size

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Biotinylated Human TSLP Protein, Ultra Low Endotoxin (TSP-HM401B-UL) Summary

Product Description

Recombinant Biotinylated Human TSLP Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr29-Gln159.

Product Background

Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.

Biotinylated Human TSLP Protein, Ultra Low Endotoxin (TSP-HM401B-UL) Specifications/Details

Protein	TSLP
Synonyms	thymic stromal lymphopoietin; TSLP
Accession	Q969D9-1
Species	Human
Biotinylated	yes
Amino Acid Range	Tyr29-Gln159
Molecular Weight	The protein has a predicted MW of 17.8 kDa. Due to furin cleavage site, the protein migrates to 12 kDa and 25-30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human TSLP, His Avi Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
Product Tag	C-His-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human TSLP, His Avi Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
	Immobilized Anti-TSLP Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TSLP, His Tag with the EC50 of 1.6ng/ml determined by ELISA.
	Biotinylated Human TSLP on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of-Biotinylated Human TSLP is greater than 95% as determined by SEC-HPLC.