Name: Biotinylated Human TSLP (R127A, R130A) Protein (TSP-HM402B)
Brand: KactusBio
SKU: TSP-HM402B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human TSLP (R127A, R130A) Protein (TSP-HM402B)

Immobilized Biotinylated Human TSLP (R127A, R130A), His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.

Immobilized Anti-TSLP Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TSLP (R127A, R130A) , His Tag with the EC50 of 3.2ng/ml determined by ELISA.

Biotinylated Human TSLP (R127A, R130A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Biotinylated Human TSLP (R127A, R130A), His-Avi Tag with an affinity constant of 4.87 nM as determined in BLI assay (Gator® Prime).

Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human TSLP (R127A, R130A), His-Avi Tag with an affinity constant of 0.22 nM as determined in BLI assay (Gator® Prime).

The purity of Biotinylated Human TSLP (R127A, R130A) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human TSLP (R127A, R130A), His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Tyr29-Gln159(R127A,R130A)

Catalog #TSP-HM402B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human TSLP (R127A, R130A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr29-Gln159(R127A,R130A).
Product Background	Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	TSLP
Synonyms	Thymic stromal lymphopoietin; TSLP
Accession	Q969D9-1
Species	Human
Biotinylated	yes
Amino Acid Range	Tyr29-Gln159(R127A,R130A)
Molecular Weight	The protein has a predicted MW of 17.7 kDa. Due to glycosylation, the protein migrates to 25-38 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human TSLP (R127A, R130A), His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human TSLP (R127A, R130A), His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.
	Immobilized Anti-TSLP Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TSLP (R127A, R130A) , His Tag with the EC50 of 3.2ng/ml determined by ELISA.
	Biotinylated Human TSLP (R127A, R130A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human TSLPR, hFc Tag on ProA-Biosensor can bind Biotinylated Human TSLP (R127A, R130A), His-Avi Tag with an affinity constant of 4.87 nM as determined in BLI assay (Gator® Prime).
	Loaded Anti-TSLP Antibody, hFc Tag on ProA-Biosensor can bind Biotinylated Human TSLP (R127A, R130A), His-Avi Tag with an affinity constant of 0.22 nM as determined in BLI assay (Gator® Prime).
	The purity of Biotinylated Human TSLP (R127A, R130A) is greater than 95% as determined by SEC-HPLC.

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