Biotinylated Human TSLP (R127A, R130A) Protein (TSP-HM402B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human TSLP (R127A, R130A) , His Tag at 1μg/ml (100μl/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 17.5ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Tyr29-Gln159(R127A,R130A)

Catalog: TSP-HM402B

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Product Description Recombinant Biotinylated Human TSLP (R127A, R130A) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Tyr29-Gln159(R127A,R130A).
Product Category Recombinant Protein
Target TSLP (R127A, R130A)
Synonyms Thymic stromal lymphopoietin; TSLP
Gene/Protein ID Q969D9-1
Species Human
Exact Sequence Tyr29-Gln159(R127A,R130A)
Molecular Weight The protein has a predicted MW of 17.7 kDa. Due to glycosylation, the protein migrates to 25-38 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated Human TSLP (R127A, R130A) , His Tag at 1μg/ml (100μl/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 17.5ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human TSLP (R127A, R130A) , His Tag at 1μg/ml (100μl/Well). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 17.5ng/ml determined by ELISA.
Immobilized Anti-TSLP Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human TSLP (R127A, R130A) , His Tag with the EC50 of 3.2ng/ml determined by ELISA.
The purity of Biotinylated Human TSLP (R127A, R130A) is greater than 95% as determined by SEC-HPLC.
Biotinylated Human TSLP (R127A, R130A) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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