Product Description |
Recombinant Biotinylated Human TSLP (R127A, R130A) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr29-Gln159(R127A,R130A). |
Product Background |
Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells. |
Product Category |
Recombinant Protein |
Target |
TSLP (R127A, R130A) |
Synonyms |
Thymic stromal lymphopoietin; TSLP |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Tyr29-Gln159(R127A,R130A) |
Molecular Weight |
The protein has a predicted MW of 17.7 kDa. Due to glycosylation, the protein migrates to 25-38 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human TSLP (R127A, R130A), His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Human TSLP (R127A, R130A), His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human TSLPR, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). |
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Immobilized Anti-TSLP Antibody, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TSLP (R127A, R130A) , His Tag with the EC50 of 3.2ng/ml determined by ELISA. |
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Biotinylated Human TSLP (R127A, R130A) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human TSLP (R127A, R130A) is greater than 95% as determined by SEC-HPLC. |
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