Product Description |
Recombinant Biotinylated Human VEGF-C/Flt4-L Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Thr103-Arg227. |
Product Background |
the lymphangiogenic factors vascular endothelial growth factor C (VEGFC) and VEGFD are cleaved by thrombin and plasmin, serine proteases generated during hemostasis and wound healing. Genetic studies reveal that platelet enhancement of lymphatic growth after wounding is dependent on the release of VEGFC, but not VEGFD, a finding consistent with high expression of VEGFC in both platelets and avian thrombocytes. |
Product Category |
Recombinant Protein |
Target |
VEGF-C/Flt4-L |
Synonyms |
VEGFC; VEGFc; Flt4-L; VRP; VEGF-C |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Thr103-Arg227 |
Molecular Weight |
The protein has a predicted MW of 17.1 kDa. Due to glycosylation, the protein migrates to 23-30 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human VEGF-C, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 50mM MES, 150mM NaCl (pH 6.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM MES, 150mM NaCl (pH 6.0). |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Human VEGF-C, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human VEGF R3, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. |
|
|
Biotinylated Human VEGF-C on Bis-Tris PAGE under reduced (R) condition. The purity is greater than 95%. |
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