Biotinylated Human VEGF R1/FLT-1 Protein (VGF-HM4R1B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Human VEGF165 at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 26.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ser27-Asn756

Catalog: VGF-HM4R1B

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Product Description Recombinant Biotinylated Human VEGF R1/FLT-1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ser27-Asn756.
Product Category Recombinant Protein
Target VEGF R1/FLT-1
Synonyms FLT; FLT1; Flt-1; FRT; VEGF R1; VEGFR1
Gene/Protein ID P17948-1
Species Human
Exact Sequence Ser27-Asn756
Molecular Weight The protein has a predicted MW of 85.1 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human VEGF165 at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 26.9ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human VEGF165 at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 26.9ng/ml determined by ELISA.
Immobilized Human PGF, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human VEGF R1, His Tag with the EC50 of 48ng/ml determined by ELISA.
Biotinylated Human VEGF R1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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