Biotinylated Human VEGF R2/KDR Protein (Primary Amine Labeling) (VGF-HM3R2B)
Biotinylated Human VEGF R2/KDR Protein (Primary Amine Labeling) (VGF-HM3R2B)
  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human VEGF R2, mFc Tag with the EC50 of 98.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala20-Glu764
Catalog #VGF-HM3R2B
Regular price $525.00
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Product Description

Recombinant Biotinylated Human VEGF R2/KDR Protein (Primary Amine Labeling) is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Ala20-Glu764.

Product Background

Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. The tyrosine kinase receptor vascular endothelial growth factor receptor 2 (VEGFR2) is a key regulator of angiogenesis.

Product Category

Recombinant Protein / Antibody discovery

Protein

VEGF R2/KDR

Synonyms

CD309; KDR; VEGFR; VEGFR2; VEGFR-21; FLK1; KRD1; Ly73

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Ala20-Glu764

Molecular Weight

The protein has a predicted MW of 110 kDa. Due to glycosylation, the protein migrates to 135-160 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human VEGF R2, mFc Tag with the EC50 of 98.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-mFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human VEGF R2, mFc Tag with the EC50 of 98.8ng/ml determined by ELISA. Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human VEGF R2, mFc Tag with the EC50 of 98.8ng/ml determined by ELISA.
Biotinylated Human VEGF R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human VEGF R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human VEGF R2 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human VEGF R2 is greater than 95% as determined by SEC-HPLC.

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