Biotinylated Human VEGF R2/KDR Protein (Primary Amine Labeling) (VGF-HM3R2B)
Biotinylated Human VEGF R2/KDR Protein (Primary Amine Labeling) (VGF-HM3R2B)
Biotinylated Human VEGF R2/KDR Protein (Primary Amine Labeling) (VGF-HM3R2B)
Biotinylated Human VEGF R2/KDR Protein (Primary Amine Labeling) (VGF-HM3R2B)
  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human VEGF R2, mFc Tag with the EC50 of 98.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala20-Glu764
Catalog #VGF-HM3R2B
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Product Description
 Recombinant Biotinylated Human VEGF R2/KDR Protein (Primary Amine Labeling) is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Ala20-Glu764.
Product Background
 Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. The tyrosine kinase receptor vascular endothelial growth factor receptor 2 (VEGFR2) is a key regulator of angiogenesis.
Product Category
 Recombinant Protein
Target
 VEGF R2/KDR
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Ala20-Glu764
Molecular Weight
 The protein has a predicted MW of 110 kDa. Due to glycosylation, the protein migrates to 135-160 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human VEGF R2, mFc Tag with the EC50 of 98.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-mFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human VEGF R2, mFc Tag with the EC50 of 98.8ng/ml determined by ELISA.
Biotinylated Human VEGF R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human VEGF R2 is greater than 95% as determined by SEC-HPLC.

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