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Biotinylated Human VEGF R2/KDR Protein, Ultra Low Endotoxin (VGF-HM4R2B-UL) Summary
Product Description |
Recombinant Biotinylated Human VEGF R2/KDR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala20-Glu764. |
Product Background |
The vascular endothelial growth factor receptor-2 (VEGFR-2/KDR/flk-1) functions as the primary mediator of vascular endothelial growth factor activation in endothelial cells. Regulation of VEGFR-2 expression appears critical in mitogenesis, differentiation, and angiogenesis. Transcriptional regulation of the VEGFR-2 is complex and may involve multiple putative upstream regulatory elements including E boxes. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets | ADC targets |
Biotinylated Human VEGF R2/KDR Protein, Ultra Low Endotoxin (VGF-HM4R2B-UL) Specifications/Details
Protein |
VEGF R2/KDR |
Synonyms |
CD309; KDR; VEGFR; VEGFR2; VEGFR-21; FLK1; KRD1; Ly73 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ala20-Glu764 |
Molecular Weight |
The protein has a predicted MW of 86.2 kDa. Due to glycosylation, the protein migrates to 120-160 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF R2, His-Avi Tag with the EC50 of 1.27ug/ml determined by ELISA. |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF R2, His-Avi Tag with the EC50 of 1.27ug/ml determined by ELISA. |
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Immobilized Biotinylated Human VEGF R2, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-VEGF R2 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. |
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Biotinylated Human VEGF R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Serial dilutions of Anti-VEGF165 Antibody were added into Human VEGF165, No Tag: Biotinylated Human VEGF R2, His Avi Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.30ug/ml. |
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The purity of Biotinylated Human VEGF R2 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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