Name: Biotinylated Human VEGF R2/KDR Protein (VGF-HM4R2B)
Brand: KactusBio
SKU: VGF-HM4R2B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human VEGF R2/KDR Protein (VGF-HM4R2B)

Immobilized Biotinylated Human VEGF R2, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-VEGF R2 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test).

Immobilized Human VEGF165, No Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human VEGF R2, HisTag with the EC50 of 2.5ug/ml determined by ELISA.

Biotinylated Human VEGF R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human VEGF R2 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human VEGF R2, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-VEGF R2 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Ala20-Glu764

Catalog #VGF-HM4R2B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human VEGF R2/KDR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala20-Glu764.
Product Background	Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. The tyrosine kinase receptor vascular endothelial growth factor receptor 2 (VEGFR2) is a key regulator of angiogenesis.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	VEGF R2/KDR
Synonyms	CD309; KDR; VEGFR; VEGFR2; VEGFR-21; FLK1; KRD1; Ly73
Accession	P35968-1
Species	Human
Biotinylated	yes
Amino Acid Range	Ala20-Glu764
Molecular Weight	The protein has a predicted MW of 86.2 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human VEGF R2, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-VEGF R2 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human VEGF R2, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-VEGF R2 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA (QC Test).
	Immobilized Human VEGF165, No Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human VEGF R2, HisTag with the EC50 of 2.5ug/ml determined by ELISA.
	Biotinylated Human VEGF R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human VEGF R2 is greater than 95% as determined by SEC-HPLC.