Biotinylated Human VEGF R2/KDR Protein (VGF-HM4R2B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-VEGF R2 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGF R2, His Tag with the EC50 of 0.35μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala20-Glu764

Catalog: VGF-HM4R2B

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Product Description Recombinant Biotinylated Human VEGF R2/KDR Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ala20-Glu764.
Product Category Recombinant Protein
Target VEGF R2/KDR
Synonyms CD309; KDR; VEGFR; VEGFR2; VEGFR-21; FLK1; KRD1; Ly73
Gene/Protein ID P35968-1
Species Human
Exact Sequence Ala20-Glu764
Molecular Weight The protein has a predicted MW of 86.2 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-VEGF R2 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGF R2, His Tag with the EC50 of 0.35μg/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-VEGF R2 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGF R2, His Tag with the EC50 of 0.35μg/ml determined by ELISA.
Immobilized Human VEGF165, No Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human VEGF R2, HisTag with the EC50 of 2.5μg/ml determined by ELISA.
The purity of Biotinylated Human VEGF R2 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human VEGF R2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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