Biotinylated Human VEGF R3/FLT4 Protein (VGF-HM4R3B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human VEGF-C, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human VEGF R3, His Tag with the EC50 of 24.3ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Tyr25-Ile776

Catalog: VGF-HM4R3B

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Product Description Recombinant Biotinylated Human VEGF R3/FLT4 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Tyr25-Ile776.
Product Category Recombinant Protein
Target VEGF R3/FLT4
Gene/Protein ID P35916-1
Species Human
Exact Sequence Tyr25-Ile776
Molecular Weight The protein has a predicted MW of 87.4 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human VEGF-C, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human VEGF R3, His Tag with the EC50 of 24.3ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human VEGF-C, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human VEGF R3, His Tag with the EC50 of 24.3ng/ml determined by ELISA.
The purity of Biotinylated Human VEGF R3 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human VEGF R3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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