Biotinylated Human VEGF R3/FLT4 Protein (VGF-HM4R3B)
Biotinylated Human VEGF R3/FLT4 Protein (VGF-HM4R3B)
Biotinylated Human VEGF R3/FLT4 Protein (VGF-HM4R3B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human VEGF-C, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human VEGF R3, His Tag with the EC50 of 23.0ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Tyr25-Ile776
Catalog #VGF-HM4R3B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human VEGF R3/FLT4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr25-Ile776.
Product Background
Vascular endothelial growth factor (VEGF) and its receptors VEGF-R1, -R2 and -R3 play important roles in tumor angiogenesis and are associated with poor prognosis in several solid tumors.VEGF-R1, -R2 and -R3 were highly expressed in CRC cells and stromal vessels. VEGF-R1 strong positive staining correlated with shorter survival after CRC surgery.
Product Category
Recombinant Protein
Target
VEGF R3/FLT4
Synonyms
VEGFR-3; FLT-4; LMPH1A; PCLFLT41; VEGFR3; FLT-41; PCL
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Tyr25-Ile776
Molecular Weight
The protein has a predicted MW of 87.4 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human VEGF-C, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human VEGF R3, His Tag with the EC50 of 23.0ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human VEGF-C, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Human VEGF R3, His Tag with the EC50 of 23.0ng/ml determined by ELISA.
Biotinylated Human VEGF R3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human VEGF R3 is greater than 95% as determined by SEC-HPLC.

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