Name: Biotinylated Human VEGF121 Protein (VEG-HM421B)
Brand: KactusBio
SKU: VEG-HM421B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human VEGF121 Protein (VEG-HM421B)

Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF121, His Tag with the EC50 of 16.5ng/ml determined by ELISA.

Biotinylated Human VEGF121 on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF121, His Tag with the EC50 of 16.5ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ala27-Arg147

Catalog #VEG-HM421B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human VEGF121 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Arg147.
Product Background	Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
Product Category	Recombinant Protein / Antibody discovery

Protein	VEGF121
Synonyms	VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609
Accession	P15692-9
Species	Human
Biotinylated	yes
Amino Acid Range	Ala27-Arg147
Molecular Weight	The protein has a predicted MW of 17 kDa. Due to glycosylation, the protein migrates to 18 kDa and 22-25 kDa under reduced (R) condition, 30 kDa and 32-40 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Activity	Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF121, His Tag with the EC50 of 16.5ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human VEGF R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF121, His Tag with the EC50 of 16.5ng/ml determined by ELISA.
	Biotinylated Human VEGF121 on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.