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Biotinylated Human VEGF165 Protein, Ultra Low Endotoxin (VEG-HM465B-UL) Summary
Product Description |
Recombinant Biotinylated Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Arg191. |
Product Background |
Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Human VEGF165 Protein, Ultra Low Endotoxin (VEG-HM465B-UL) Specifications/Details
Protein |
VEGF165 |
Synonyms |
VEGF; VEGFA; VEGF165a; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Ala27-Arg191 |
Molecular Weight |
The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reduced (N) condition based on Bis-Tris PAGE result. |
Activity |
Immobilized Human VEGF R1, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 10.5ng/ml determined by ELISA. The affinity constant of 1.36 pM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 90% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Biotinylated Human VEGF165, His Tag captured on CM5 Chip via streptavidin can bind Human VEGF R1, His Tag with an affinity constant of 1.36 pM as determined in SPR assay (Biacore T200). |
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Immobilized Human VEGF R1, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 10.5ng/ml determined by ELISA. |
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Immobilized Anti-VEGF165 Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 11.6ng/ml determined by ELISA. |
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Immobilized Human VEGF R2, hFc Tag at 2ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 12.6ng/ml determined by ELISA. |
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Biotinylated VEGF165 on Bis-Tris PAGE under reduced (R) condition and Non reduced (N) condition. The purity is greater than 90%. |
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The purity of Biotinylated Human VEGF165 is greater than 90% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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