Name: Biotinylated Human VEGF165 Protein (VEG-HM465B)
Brand: KactusBio
SKU: VEG-HM465B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Human VEGF165 Protein (VEG-HM465B)

Biotinylated Human VEGF165, His Tag captured on CM5 Chip via streptavidin can bind Human VEGF R1, His Tag with an affinity constant of 1.36 pM as determined in SPR assay (Biacore T200).

Immobilized Anti-VEGF165 Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 11.6ng/ml determined by ELISA.

Immobilized Human VEGF R2, hFc Tag at 2ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 12.6ng/ml determined by ELISA.

Immobilized Human VEGF R1, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 10.5ng/ml determined by ELISA.

Biotinylated VEGF165 on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Anti-VEGF165 Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 11.6ng/ml determined by ELISA. The affinity constant of 1.36 pM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ala27-Arg191

Catalog #VEG-HM465B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human VEGF165 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Arg191.
Product Background	Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189.
Product Category	Recombinant Protein / Antibody discovery

Protein	VEGF165
Synonyms	VEGF; VEGFA; VEGF165a; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609;VEGF-165
Accession	P15692-4
Species	Human
Biotinylated	yes
Amino Acid Range	Ala27-Arg191
Molecular Weight	The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa under reduced (R) condition, 45-60 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Activity	Immobilized Anti-VEGF165 Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 11.6ng/ml determined by ELISA. The affinity constant of 1.36 pM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Biotinylated Human VEGF165, His Tag captured on CM5 Chip via streptavidin can bind Human VEGF R1, His Tag with an affinity constant of 1.36 pM as determined in SPR assay (Biacore T200).
	Immobilized Anti-VEGF165 Antibody at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 11.6ng/ml determined by ELISA.
	Immobilized Human VEGF R2, hFc Tag at 2ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 12.6ng/ml determined by ELISA.
	Immobilized Human VEGF R1, His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 10.5ng/ml determined by ELISA.
	Biotinylated VEGF165 on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.