Biotinylated Human VEGF165 Protein (VEG-HM465B)
Catalog: VEG-HM465B
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| Product Description | Recombinant Biotinylated Human VEGF165 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ala27-Arg191. |
| Product Category | Recombinant Protein |
| Target | VEGF165 |
| Synonyms | VEGF; VEGFA; MVCD1; VAS; VEGFMGC70609; VPF; RP1-261G23.1; MGC70609 |
| Gene/Protein ID | P15692-4 |
| Species | Human |
| Exact Sequence | Ala27-Arg191 |
| Molecular Weight | The protein has a predicted MW of 22.2 kDa. Due to glycosylation, the protein migrates to 28-32 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Anti-VEGF165 Antibody at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 32.8ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Anti-VEGF165 Antibody at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human VEGF165, His Tag with the EC50 of 32.8ng/ml determined by ELISA. |
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The purity of Biotinylated Human VEGF165 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated VEGF165 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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