Biotinylated Mouse ANGPTL3/Angiopoietin-like 3 Protein (ANG-MM403B)

Immobilized Biotinylated Mouse ANGPTL3, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA.

Biotinylated Mouse ANGPTL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Mouse ANGPTL3 is greater than 90% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Biotinylated Mouse ANGPTL3, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA.
Amino Acid Range: Ser17-Thr455

Catalog #ANG-MM403B

Price

$578⁰⁰

Size

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Biotinylated Mouse ANGPTL3/Angiopoietin-like 3 Protein (ANG-MM403B) Summary

Product Description

Recombinant Biotinylated Mouse ANGPTL3/Angiopoietin-like 3 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ser17-Thr455.

Product Background

ANGPTL3 is a secreted glycoprotein that is structurally related to the angiopoietins. Mature human ANGPTL3 contains an N-terminal coiled coil domain and a C‑terminal fibrinogen-like domain. ANGPTL3 is expressed in the liver from early in development through adulthood. Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism. Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake.

Biotinylated Mouse ANGPTL3/Angiopoietin-like 3 Protein (ANG-MM403B) Specifications/Details

Protein	ANGPTL3/Angiopoietin-like 3
Synonyms	ANGPTL3; ANGPT5; ANG-5; Angiopoietin-5; FHBL2; ANL3; Angiopoietin-5; Angiopoietin-like Protein 3;ANG5;Angiopoietin 5; ANGPT-5; ANGPT 5
Accession	Q9R182
Species	Mouse
Biotinylated	yes
Amino Acid Range	Ser17-Thr455
Molecular Weight	The protein has a predicted MW of 54.14 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Mouse ANGPTL3, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA.
Product Tag	C-His-Avi
Fluorophore	Biotin-labeled
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 0.05% CHAPS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Mouse ANGPTL3, His-Avi Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-ANGPTL3 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA.
	Biotinylated Mouse ANGPTL3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Mouse ANGPTL3 is greater than 90% as determined by SEC-HPLC.