Product Description |
Recombinant Biotinylated Mouse APLN Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Val23-Phe77. |
Product Background |
Macrophages play key roles during cardiovascular diseases (CVD) and their related complications.Apelin (APLN) is a key molecule, whose roles during CVD have been documented previously. Therefore, it has been hypothesized that APLN may perform its roles via modulation of macrophages. |
Product Category |
Recombinant Protein |
Target |
APLN |
Accession |
|
Species |
Mouse |
Biotinylated |
yes |
Amino Acid Range |
Val23-Phe77 |
Molecular Weight |
The protein has a predicted MW of 34.81 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS, 0.2M Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Biotinylated Mouse APLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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