Biotinylated Mouse CDCP1 Protein (CDC-MM401B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: The affinity constant of 0.45 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Arg25-Ala667

Catalog: CDC-MM401B

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Product Description Recombinant Biotinylated Mouse CDCP1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg25-Ala667.

Product Background

Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.

Product Category

Recombinant Protein

Target

CDCP1
Synonyms CDCP1; CD318; SIMA135; TRASK; UNQ2486; PRO5773

Gene/Protein ID

Q5U462

Species

Mouse

Exact Sequence

Arg25-Ala667
Molecular Weight The protein has a predicted MW of 75.3 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 0.45 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-CDCP1 Antibody captured on CM5 Chip via Protein A can bind Biotinylated Mouse CDCP-1, His Tag with an affinity constant of 0.45 uM as determined in SPR assay (Biacore T200).
The purity of Biotinylated Mouse CDCP1 is greater than 95% as determined by SEC-HPLC.
Biotinylated Mouse CDCP1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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