Name: Biotinylated Mouse Fc gamma RIII/CD16 Protein (FGR-MM4R3B)
Brand: KactusBio
SKU: FGR-MM4R3B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Mouse Fc gamma RIII/CD16 Protein (FGR-MM4R3B)

OKT3 captured on CM5 Chip via Protein A can bind Biotinylated Mouse Fc gamma RIII, His Tag with an affinity constant of 0.164 uM as determined in SPR assay (Biacore T200).

Biotinylated Mouse Fc gamma RIII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Mouse Fc gamma RIII is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: The affinity constant of 0.164 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Leu32-Thr215

Catalog #FGR-MM4R3B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Mouse Fc gamma RIII/CD16 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Leu32-Thr215.
Product Background	Immunoglobulin G (IgG) Fc receptors play a critical role in linking IgG antibody-mediated immune responses with cellular effector functions. A high resolution map of the binding site on human IgG1 for human Fc gamma RI, Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIIA, and FcRn receptors has been determined.A common set of IgG1 residues is involved in binding to all Fc gamma R; Fc gamma RII and Fc gamma RIII also utilize residues outside this common set.
Product Category	Recombinant Protein / Antibody discovery / Fc receptors

Protein	Fc gamma RIII/CD16
Synonyms	IgG Fc receptor III; Fc-gamma RIII; FcRIII; FCGR3; CD16; CD16A; FCG3; FcgRIII; FCR-10; FCRIIIA; IGFR3; IMD20; FcγRIII; Fc γRIII; Fcγ RIII; Fc γ RIII
Accession	Q5D5I8
Species	Mouse
Biotinylated	yes
Amino Acid Range	Leu32-Thr215
Molecular Weight	The protein has a predicted MW of 23.97 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.164 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	OKT3 captured on CM5 Chip via Protein A can bind Biotinylated Mouse Fc gamma RIII, His Tag with an affinity constant of 0.164 uM as determined in SPR assay (Biacore T200).
	Biotinylated Mouse Fc gamma RIII on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Mouse Fc gamma RIII is greater than 95% as determined by SEC-HPLC.