Biotinylated Mouse Fc gamma RIII/CD16 Protein (FGR-MM4R3B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: The affinity constant of 0.164 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Leu32-Thr215

Catalog: FGR-MM4R3B

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Product Description Recombinant Biotinylated Mouse Fc gamma RIII/CD16 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Leu32-Thr215.

Product Background

Immunoglobulin G (IgG) Fc receptors play a critical role in linking IgG antibody-mediated immune responses with cellular effector functions. A high resolution map of the binding site on human IgG1 for human Fc gamma RI, Fc gamma RIIA, Fc gamma RIIB, Fc gamma RIIIA, and FcRn receptors has been determined.A common set of IgG1 residues is involved in binding to all Fc gamma R; Fc gamma RII and Fc gamma RIII also utilize residues outside this common set.

Product Category

Recombinant Protein

Target

Fc gamma RIII/CD16
Accession

Species

Mouse

Exact Sequence

Leu32-Thr215
Molecular Weight The protein has a predicted MW of 23.97 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 0.164 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
OKT3 captured on CM5 Chip via Protein A can bind Biotinylated Mouse Fc gamma RIII, His Tag with an affinity constant of 0.164 uM as determined in SPR assay (Biacore T200).
The purity of Biotinylated Mouse Fc gamma RIII is greater than 95% as determined by SEC-HPLC.
Biotinylated Mouse Fc gamma RIII on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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