Product Description |
Recombinant Biotinylated Mouse FGL2 Protein is expressed from HEK293 with His tag and Avi tag and Flag tag at the N-Terminus.It contains Pro197-Pro432. |
Product Background |
Fibrinogen-like protein 2 (FGL2) is a member of the fibrinogen-like protein family and possesses important regulatory functions in both innate and adaptive immune responses. FGL2 is overexpressed in glioma, and its expression level is negatively associated with the prognosis of glioma patients. |
Product Category |
Recombinant Protein |
Target |
FGL2 |
Synonyms |
Fibroleukin; pT49; FGL2; fibrinogen-like 2; |
Accession |
|
Species |
Mouse |
Biotinylated |
yes |
Amino Acid Range |
Pro197-Pro432 |
Molecular Weight |
The protein has a predicted MW of 31.48 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Mouse FGL2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His-Avi,N-Flag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated Mouse FGL2, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-FGL2 Antibody, hFc Tag with the EC50 of 8.1ng/ml determined by ELISA. |
|
|
Biotinylated Mouse FGL2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Biotinylated Mouse FGL2 is greater than 95% as determined by SEC-HPLC. |
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