Name: Biotinylated Mouse GFRAL/GFR alpha-like Protein (GFL-MM401B)
Brand: KactusBio
SKU: GFL-MM401B-100UG
Price: 660.0 USD
Availability: InStock

Biotinylated Mouse GFRAL/GFR alpha-like Protein (GFL-MM401B)

Immobilized Biotinylated Mouse GFRAL, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.

Biotinylated Mouse GFRAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Mouse GFRAL is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Mouse GFRAL, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln20-Glu350

Catalog #GFL-MM401B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Mouse GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gln20-Glu350.
Product Background	GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	GFRAL/GFR alpha-like
Synonyms	GFR alpha-like; GFRAL; GRAL; C6orf144
Accession	Q6SJE0-1
Species	Mouse
Biotinylated	yes
Amino Acid Range	Gln20-Glu350
Molecular Weight	The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Mouse GFRAL, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Mouse GFRAL, His Tag at 0.5ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 2.4ng/ml determined by ELISA.
	Biotinylated Mouse GFRAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Mouse GFRAL is greater than 95% as determined by SEC-HPLC.