Product Description |
Recombinant Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His24-Pro297 (H-2K(b)), Ile21-Met119 (B2M) and SIINFEKL peptide. |
Product Background |
Ovalbumin (OVA) has been historically a popular source of such antigens, since OVA can induce both humoral and cellular immune responses based on well-characterised peptide epitopes. The OVA257-264 octapeptide was one of the frst OVA epitopes to be characterised, it has an amino acid sequence SIINFEKL, which is recognised by cytotoxic T lymphocytes. SIINFEKL forms fbrillar assemblies similar to other peptide hydrogels. Te immunoactive properties of this peptide can therefore be related to its self-assembling nature. |
Product Category |
Recombinant Protein |
Target |
H-2K(b)&B2M&OVA (SIINFEKL) Monomer |
Synonyms |
H-2K; OVA; MHC; H2-K1; |
MHC Form |
Monomer |
Accession |
P01901(H-2K(b))&P01887(B2M)&SIINFEKL |
Species |
Mouse |
Biotinylated |
yes |
Amino Acid Range |
His24-Pro297(H-2K(b)), Ile21-Met119(B2M) and SIINFEKL peptide |
Molecular Weight |
The protein has a predicted MW of 50.20 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein is greater than 95% as determined by SEC-HPLC. |
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