Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein (MHC-MM453B)
Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein (MHC-MM453B)
Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein (MHC-MM453B)
Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein (MHC-MM453B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: His24-Pro297(H-2K(b)), Ile21-Met119(B2M) and SIINFEKL peptide
Catalog #MHC-MM453B
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Product Description
 Recombinant Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His24-Pro297 (H-2K(b)), Ile21-Met119 (B2M) and SIINFEKL peptide.
Product Background
 Ovalbumin (OVA) has been historically a popular source of such antigens, since OVA can induce both humoral and cellular immune responses based on well-characterised peptide epitopes. The OVA257-264 octapeptide was one of the frst OVA epitopes to be characterised, it has an amino acid sequence SIINFEKL, which is recognised by cytotoxic T lymphocytes. SIINFEKL forms fbrillar assemblies similar to other peptide hydrogels. Te immunoactive properties of this peptide can therefore be related to its self-assembling nature.
Product Category
 Recombinant Protein
Target
 H-2K(b)&B2M&OVA (SIINFEKL) Monomer
MHC Form
 Monomer
Accession
Species
 Mouse
Biotinylated
 yes
Amino Acid Range
 His24-Pro297(H-2K(b)), Ile21-Met119(B2M) and SIINFEKL peptide
Molecular Weight
 The protein has a predicted MW of 50.20 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse H-2K(b)&B2M&OVA (SIINFEKL) Monomer Protein is greater than 95% as determined by SEC-HPLC.
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