Biotinylated Mouse IFN alpha/beta R2 Protein (IFN-MM1R2B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 24.31 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ser22-Ala242
Catalog #IFN-MM1R2B
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Product Description
 Recombinant Biotinylated Mouse IFN alpha/beta R2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ser22-Ala242.
Product Background
 Although interferon (IFN)-α is known to exert immunomodulatory and antiproliferative effects on dendritic cells (DCs) through induction of protein-coding IFN-stimulated genes (ISGs), little is known about IFN-α-regulated miRNAs in DCs. Since several miRNAs are involved in regulating DC functions, it is important to investigate whether IFN-α's effects on DCs are mediated through miRNAs as well.
Product Category
 Recombinant Protein
Target
 IFN alpha/beta R2
Accession
Species
 Mouse
Biotinylated
 yes
Amino Acid Range
 Ser22-Ala242
Molecular Weight
 The protein has a predicted MW of 28.34 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 24.31 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Mouse IFN alpha/beta R2, His-Avi Tag captured on CM5 Chip via anti-his antibody can bind Mouse IFN alpha 1, hFc Tag with an affinity constant of 24.31 nM as determined in SPR assay (Biacore T200).
Biotinylated Mouse IFN alpha/beta R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse IFN alpha/beta R2 is greater than 95% as determined by SEC-HPLC.

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