Biotinylated Mouse IFN alpha/beta R2 Protein (IFN-MM1R2B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 24.31 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ser22-Ala242

Catalog: IFN-MM1R2B

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Product Description Recombinant Biotinylated Mouse IFN alpha/beta R2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ser22-Ala242.

Product Background

Although interferon (IFN)-α is known to exert immunomodulatory and antiproliferative effects on dendritic cells (DCs) through induction of protein-coding IFN-stimulated genes (ISGs), little is known about IFN-α-regulated miRNAs in DCs. Since several miRNAs are involved in regulating DC functions, it is important to investigate whether IFN-α's effects on DCs are mediated through miRNAs as well.

Product Category

Recombinant Protein

Target

IFN alpha/beta R2

Gene/Protein ID

O35664-1

Species

Mouse

Exact Sequence

Ser22-Ala242
Molecular Weight The protein has a predicted MW of 28.34 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

Activity

The affinity constant of 24.31 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Mouse IFN alpha/beta R2, His-Avi Tag captured on CM5 Chip via anti-his antibody can bind Mouse IFN alpha 1, hFc Tag with an affinity constant of 24.31 nM as determined in SPR assay (Biacore T200).
The purity of Biotinylated Mouse IFN alpha/beta R2 is greater than 95% as determined by SEC-HPLC.
Biotinylated Mouse IFN alpha/beta R2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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