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Biotinylated Mouse IFN alpha/beta R2 Protein (IFN-MM1R2B) Summary
Product Description |
Recombinant Biotinylated Mouse IFN alpha/beta R2 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Ser22-Ala242. |
Product Background |
Although interferon (IFN)-α is known to exert immunomodulatory and antiproliferative effects on dendritic cells (DCs) through induction of protein-coding IFN-stimulated genes (ISGs), little is known about IFN-α-regulated miRNAs in DCs. Since several miRNAs are involved in regulating DC functions, it is important to investigate whether IFN-α's effects on DCs are mediated through miRNAs as well. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Biotinylated Mouse IFN alpha/beta R2 Protein (IFN-MM1R2B) Specifications/Details
Protein |
IFN alpha/beta R2 |
Synonyms |
IFN-R-2; IFNAR2; IFNARB; IFNABR; IFN-alpha; IFN-alpha/beta R2; IFNbR2; IFN-R; β receptor 2; β receptor10; |
Accession |
|
Species |
Mouse |
Biotinylated |
yes |
Amino Acid Range |
Ser22-Ala242 |
Molecular Weight |
The protein has a predicted MW of 28.34 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 24.31 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Fluorophore |
Biotin-labeled |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Biotinylated Mouse IFN alpha/beta R2, His-Avi Tag captured on CM5 Chip via anti-his antibody can bind Mouse IFN alpha 1, hFc Tag (Cat. IFN-MM2A1) with an affinity constant of 24.31 nM as determined in SPR assay (Biacore T200). |
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Biotinylated Mouse IFN alpha/beta R2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Mouse IFN alpha/beta R2 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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