Biotinylated Mouse IL-1 Beta/IL-1F2 Protein (IL1-ME40BB)
Biotinylated Mouse IL-1 Beta/IL-1F2 Protein (IL1-ME40BB)
  • Express System: E.coli
  • Product Tag: N-His-Avi
  • Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
  • Activity: Immobilized Biotinylated Mouse IL-1 Beta, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-1 R2, hFc Tag with the EC50 of 25.4ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Val118-Ser269
Catalog #IL1-ME40BB
Regular price $525.00
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Product Description
Recombinant Biotinylated Mouse IL-1 Beta/IL-1F2 Protein is expressed from E.coli with His tag and Avi tag at the N-terminus.It contains Val118-Ser269.
Product Background
Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells.
Product Category
Recombinant Protein
Target
IL-1 Beta/IL-1F2
Accession
Species
Mouse
Biotinylated
yes
Amino Acid Range
Val118-Ser269
Molecular Weight
The protein has a predicted MW of 20.30 kDa. The protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Mouse IL-1 Beta, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-1 R2, hFc Tag with the EC50 of 25.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
N-His-Avi
Express System
E.coli
Purity
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Mouse IL-1 Beta, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-1 R2, hFc Tag with the EC50 of 25.4ng/ml determined by ELISA (QC Test).
Immobilized Mouse IL-1R2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-1 Beta, His Tag with the EC50 of 1.2ug/ml determined by ELISA.
Biotinylated Mouse IL-1 Beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse IL-1 Beta is greater than 95% as determined by SEC-HPLC.

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