Name: Biotinylated Mouse IL-1 Beta/IL-1F2 Protein (IL1-ME40BB)
Brand: KactusBio
SKU: IL1-ME40BB-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Mouse IL-1 Beta/IL-1F2 Protein (IL1-ME40BB)

Immobilized Biotinylated Mouse IL-1 Beta, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-1 R2, hFc Tag with the EC50 of 25.4ng/ml determined by ELISA (QC Test).

Immobilized Mouse IL-1R2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-1 Beta, His Tag with the EC50 of 1.2ug/ml determined by ELISA.

Biotinylated Mouse IL-1 Beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Mouse IL-1 Beta is greater than 95% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: N-His-Avi
Purity: >95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Activity: Immobilized Biotinylated Mouse IL-1 Beta, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-1 R2, hFc Tag with the EC50 of 25.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Val118-Ser269

Catalog #IL1-ME40BB

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Mouse IL-1 Beta/IL-1F2 Protein is expressed from E.coli with His tag and Avi tag at the N-terminus.It contains Val118-Ser269.
Product Background	Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells.
Product Category	Recombinant Protein / Antibody discovery

Protein	IL-1 Beta/IL-1F2
Synonyms	Interleukin-1 beta; IL-1 beta; IL1F2; IL1B; IL-1BETA; IL1F2; IL-1β; IL-1 beta; IL-1B ;Interleukin-1 β; IL-1 β; IL-1β; IL-1 β
Accession	P10749
Species	Mouse
Biotinylated	yes
Amino Acid Range	Val118-Ser269
Molecular Weight	The protein has a predicted MW of 20.30 kDa. The protein migrates to 20-25 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Mouse IL-1 Beta, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-1 R2, hFc Tag with the EC50 of 25.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-His-Avi
Expression System	E.coli
Purity	>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Mouse IL-1 Beta, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-1 R2, hFc Tag with the EC50 of 25.4ng/ml determined by ELISA (QC Test).
	Immobilized Mouse IL-1R2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-1 Beta, His Tag with the EC50 of 1.2ug/ml determined by ELISA.
	Biotinylated Mouse IL-1 Beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Mouse IL-1 Beta is greater than 95% as determined by SEC-HPLC.