Product Description |
Recombinant Biotinylated Mouse IL-1 Beta/IL-1F2 Protein is expressed from E.coli with His tag and Avi tag at the N-terminus.It contains Val118-Ser269. |
Product Background |
Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells. |
Product Category |
Recombinant Protein |
Target |
IL-1 Beta/IL-1F2 |
Accession |
|
Species |
Mouse |
Biotinylated |
yes |
Amino Acid Range |
Val118-Ser269 |
Molecular Weight |
The protein has a predicted MW of 20.30 kDa. The protein migrates to 20-25 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Mouse IL-1 Beta, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-1 R2, hFc Tag with the EC50 of 25.4ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-His-Avi |
Express System |
E.coli |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Biotinylated Mouse IL-1 Beta, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-1 R2, hFc Tag with the EC50 of 25.4ng/ml determined by ELISA (QC Test). |
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Immobilized Mouse IL-1R2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-1 Beta, His Tag with the EC50 of 1.2ug/ml determined by ELISA. |
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Biotinylated Mouse IL-1 Beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Mouse IL-1 Beta is greater than 95% as determined by SEC-HPLC. |
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