Biotinylated Mouse IL-17A/CTLA-8 Protein (ILA-MM417B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Mouse IL-17R alpha at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse IL-17A, His Tag with the EC50 of 14.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala26-Ala158

Catalog: ILA-MM417B

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Product Description Recombinant Biotinylated Mouse IL-17A/CTLA-8 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ala26-Ala158.
Product Category Recombinant Protein
Target IL-17A/CTLA-8
Synonyms CTLA-8; IL17; IL17A; IL-17CTLA-8; interleukin 17A
Gene/Protein ID Q62386-1
Species Mouse
Exact Sequence Ala26-Ala158
Molecular Weight The protein has a predicted MW of 18 kDa. Due to glycosylation, the protein migrates to 19-29 kDa based on Tris-Bis PAGE result.
Activity Immobilized Mouse IL-17R alpha at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse IL-17A, His Tag with the EC50 of 14.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse IL-17R alpha at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse IL-17A, His Tag with the EC50 of 14.7ng/ml determined by ELISA.
Biotinylated Mouse IL-17A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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