Biotinylated Mouse IL-17F Protein (ILF-MM417B)
Biotinylated Mouse IL-17F Protein (ILF-MM417B)
Biotinylated Mouse IL-17F Protein (ILF-MM417B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Mouse IL-17RA , hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse IL-17F, His Tag with the EC50 of 1.75ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Arg29-Ala161
Catalog #ILF-MM417B
Regular price $525.00
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Product Description
Recombinant Biotinylated Mouse IL-17F Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg29-Ala161.
Product Background
The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N‑terminus.IL-17F is ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover.
Product Category
Recombinant Protein
Target
IL-17F
Synonyms
Cytokine ML-1; IL17F; IL-17F; IL24; IL-24; CANDF6; IL-17F; ML1
Accession
Species
Mouse
Biotinylated
yes
Amino Acid Range
Arg29-Ala161
Molecular Weight
The protein has a predicted MW of 17.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse IL-17RA , hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse IL-17F, His Tag with the EC50 of 1.75ug/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse IL-17RA , hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse IL-17F, His Tag with the EC50 of 1.75ug/ml determined by ELISA.
Biotinylated Mouse IL-17F on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse IL-17F is greater than 95% as determined by SEC-HPLC.

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