Product Description |
Recombinant Biotinylated Mouse IL-17F Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg29-Ala161. |
Product Background |
The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N‑terminus.IL-17F is ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover. |
Product Category |
Recombinant Protein |
Target |
IL-17F |
Synonyms |
Cytokine ML-1; IL17F; IL-17F; IL24; IL-24; CANDF6; IL-17F; ML1 |
Accession |
|
Species |
Mouse |
Biotinylated |
yes |
Amino Acid Range |
Arg29-Ala161 |
Molecular Weight |
The protein has a predicted MW of 17.8 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse IL-17RA , hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse IL-17F, His Tag with the EC50 of 1.75ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Mouse IL-17RA , hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse IL-17F, His Tag with the EC50 of 1.75ug/ml determined by ELISA. |
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Biotinylated Mouse IL-17F on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Mouse IL-17F is greater than 95% as determined by SEC-HPLC. |
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