Biotinylated Mouse IL-2 Protein (IL2-MM401B)

  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Mouse IL-2 R alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 65.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala21-Gln169

Catalog: IL2-MM401B

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Product Description Recombinant Biotinylated Mouse IL-2 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Ala21-Gln169.
Product Category Recombinant Protein
Target IL-2
Synonyms IL2; T-cell growth factor; TCGF; Aldesleukin
Gene/Protein ID P04351-1
Species Mouse
Exact Sequence Ala21-Gln169
Molecular Weight The protein has a predicted MW of 20.1 kDa. Due to glycosylation, the protein migrates to 27-30 kDa based on Tris-Bis PAGE result.
Activity Immobilized Mouse IL-2 R alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 65.2ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Mouse IL-2 R alpha, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 65.2ng/ml determined by ELISA.
The purity of Biotinylated Mouse IL-2 is greater than 95% as determined by SEC-HPLC.
Biotinylated Mouse IL-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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