Biotinylated Mouse IL-21 Protein (IL2-ME421B)
Biotinylated Mouse IL-21 Protein (IL2-ME421B)
  • Express System: E.coli
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human IL-21R, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-21, His Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: His18-Ser146
Catalog #IL2-ME421B
Regular price $525.00
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Product Description
Recombinant Biotinylated Mouse IL-21 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains His18-Ser146.
Product Background
Interleukin-21 (IL-21), produced predominantly by CD4 T cells and natural killer T (NKT) cells, is a newly discovered member of the common γ-chain family of cytokines. It has been implicated in many immunological processes and has been linked to autoimmune diseases, allergies and other inflammatory diseases.
Product Category
Recombinant Protein
Target
IL-21
Synonyms
Interleukin-21; IL-21; Za11; CVID11
Accession
Species
Mouse
Biotinylated
yes
Amino Acid Range
His18-Ser146
Molecular Weight
The protein has a predicted MW of 17.9 kDa same as Bis-Tris PAGE result.
Activity
Immobilized Human IL-21R, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-21, His Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
N-His-Avi
Express System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 20mM NaAc, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.5).
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human IL-21R, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-21, His Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test).
Biotinylated Mouse IL-21 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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