- Express System: E.coli
- Product Tag: N-His-Avi
- Purity: > 95% as determined by Bis-Tris PAGE
- Activity: Immobilized Human IL-21R, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-21, His Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). See testing image for detail.
- Amino Acid Range: His18-Ser146
Biotinylated Mouse IL-21 Protein (IL2-ME421B) Summary
Product Description |
Recombinant Biotinylated Mouse IL-21 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains His18-Ser146. |
Product Background |
Interleukin-21 (IL-21), produced predominantly by CD4 T cells and natural killer T (NKT) cells, is a newly discovered member of the common γ-chain family of cytokines. It has been implicated in many immunological processes and has been linked to autoimmune diseases, allergies and other inflammatory diseases. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Biotinylated Mouse IL-21 Protein (IL2-ME421B) Specifications/Details
Protein |
IL-21 |
Synonyms |
Interleukin-21; IL-21; Za11; CVID11 |
Accession |
|
Species |
Mouse |
Biotinylated |
yes |
Amino Acid Range |
His18-Ser146 |
Molecular Weight |
The protein has a predicted MW of 17.9 kDa same as Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-21R, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse IL-21, His Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-His-Avi |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20mM NaAc, 150mM NaCl (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.5). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
More from Antibody Drug Discovery Proteins
Recently viewed