Biotinylated Mouse IL-22 Protein (IL2-ME422B)

  • Express System: E.coli
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA. The affinity constant of 0.145 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Leu34-Val179

Catalog: IL2-ME422B

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Product Description Recombinant Biotinylated Mouse IL-22 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Leu34-Val179.

Product Background

The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production.

Product Category

Recombinant Protein

Target

IL-22
Synonyms Interleukin-22; IL-22; Cytokine Zcyto18; IL-TIF; IL-D110; TIFa; TIFIL-23; ZCYTO18

Gene/Protein ID

Q9JJY9

Species

Mouse

Exact Sequence

Leu34-Val179
Molecular Weight The protein has a predicted MW of 19.7 kDa same as Tris-Bis PAGE result.

Activity

Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA. The affinity constant of 0.145 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

N-His-Avi

Express System

E.coli
Purity > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Rat IL-22R alpha 1, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-22, His Tag with an affinity constant of 0.145 uM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA.
The purity of Biotinylated Mouse IL-22 is greater than 90% as determined by SEC-HPLC.
Biotinylated Mouse IL-22 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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