Biotinylated Mouse IL-22 Protein (IL2-ME422B)

Express System: E.coli
Product Tag: N-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Biotinylated: yes
Activity: Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA. The affinity constant of 0.145 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Leu34-Val179

Catalog: IL2-ME422B

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Product Description	Recombinant Biotinylated Mouse IL-22 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Leu34-Val179.
Product Background	The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production.
Product Category	Recombinant Protein

Target	IL-22
Synonyms	Interleukin-22; IL-22; Cytokine Zcyto18; IL-TIF; IL-D110; TIFa; TIFIL-23; ZCYTO18
Gene/Protein ID	Q9JJY9
Species	Mouse
Exact Sequence	Leu34-Val179
Molecular Weight	The protein has a predicted MW of 19.7 kDa same as Tris-Bis PAGE result.
Activity	Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA. The affinity constant of 0.145 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Avi
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Rat IL-22R alpha 1, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-22, His Tag with an affinity constant of 0.145 uM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA.
	The purity of Biotinylated Mouse IL-22 is greater than 90% as determined by SEC-HPLC.
	Biotinylated Mouse IL-22 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.