Biotinylated Mouse IL-22 Protein (IL2-ME422B)
Biotinylated Mouse IL-22 Protein (IL2-ME422B)
Biotinylated Mouse IL-22 Protein (IL2-ME422B)
Biotinylated Mouse IL-22 Protein (IL2-ME422B)
  • Express System: E.coli
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.145 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu34-Val179
Catalog #IL2-ME422B
Regular price $525.00
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Product Description
Recombinant Biotinylated Mouse IL-22 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Leu34-Val179.
Product Background
The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production.
Product Category
Recombinant Protein
Target
IL-22
Synonyms
Interleukin-22; IL-22; Cytokine Zcyto18; IL-TIF; IL-D110; TIFa; TIFIL-23; ZCYTO18
Accession
Species
Mouse
Biotinylated
yes
Amino Acid Range
Leu34-Val179
Molecular Weight
The protein has a predicted MW of 19.7 kDa same as Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.145 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
N-His-Avi
Express System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Rat IL-22R alpha 1, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-22, His Tag with an affinity constant of 0.145 uM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA (QC Test).
Biotinylated Mouse IL-22 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse IL-22 is greater than 90% as determined by SEC-HPLC.

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