Name: Biotinylated Mouse IL-22 Protein (IL2-ME422B)
Brand: KactusBio
SKU: IL2-ME422B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Mouse IL-22 Protein (IL2-ME422B)

Rat IL-22R alpha 1, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-22, His Tag with an affinity constant of 0.145 uM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA (QC Test).

Biotinylated Mouse IL-22 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Mouse IL-22R alpha 1, hFc Tag on ProA-Biosensor can bind Biotinylated Mouse IL-22, His-Avi Tag with an affinity constant of 13.50 nM as determined in BLI assay (Gator® Prime).

The purity of Biotinylated Mouse IL-22 is greater than 90% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.145 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Leu34-Val179

Catalog #IL2-ME422B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Mouse IL-22 Protein (IL2-ME422B) Summary

Product Description	Recombinant Biotinylated Mouse IL-22 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Leu34-Val179.
Product Background	The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Mouse IL-22 Protein (IL2-ME422B) Specifications/Details

Protein	IL-22
Synonyms	Interleukin-22; IL-22; Cytokine Zcyto18; IL-TIF; IL-D110; TIFa; TIFIL-23; ZCYTO18
Accession	Q9JJY9
Species	Mouse
Biotinylated	yes
Amino Acid Range	Leu34-Val179
Molecular Weight	The protein has a predicted MW of 19.7 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.145 uM as determined in SPR assay (Biacore T200).
Product Tag	N-His-Avi
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Rat IL-22R alpha 1, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-22, His Tag with an affinity constant of 0.145 uM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Mouse IL-22, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA (QC Test).
	Biotinylated Mouse IL-22 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Mouse IL-22R alpha 1, hFc Tag on ProA-Biosensor can bind Biotinylated Mouse IL-22, His-Avi Tag with an affinity constant of 13.50 nM as determined in BLI assay (Gator® Prime).
	The purity of Biotinylated Mouse IL-22 is greater than 90% as determined by SEC-HPLC.