Name: Biotinylated Mouse IL-5 Protein (IL5-MM401B)
Brand: KactusBio
SKU: IL5-MM401B-100UG
Price: 577.5 USD
Availability: InStock

Surface plasmon resonance (SPR) binding kinetics curve for biotinylated recombinant mouse IL-5 protein, showing response units (RU) vs. concentration. Graph demonstrates IL-5 ligand-receptor interaction and binding affinity.

Mouse IL-5 R alpha, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-5, His-Avi Tag with an affinity constant of 0.511 uM as determined in SPR assay (Biacore T200).

Biotinylated Mouse IL-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: The affinity constant of 0.511 uM as determined in SPR assay (Biacore T200).
Amino Acid Range: Met21-Gly133
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Catalog #IL5-MM401B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Mouse IL-5 Protein (IL5-MM401B) Summary

Product Description	Recombinant Biotinylated Mouse IL-5 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Met21-Gly133.
Product Background	IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(Rα) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA).
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Mouse IL-5 Protein (IL5-MM401B) Specifications/Details

Protein	IL-5
Synonyms	Interleukin-5; IL-5; T-cell replacing factor; TRF; EDF
Accession	P04401
Species	Mouse
Biotinylated	yes
Amino Acid Range	Met21-Gly133
Molecular Weight	The protein has a predicted MW of 16.03 kDa. Due to glycosylation, the protein migrates to 16 kDa and 20-35 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.511 uM as determined in SPR assay (Biacore T200).
Product Tag	N-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22um filtered solution in 20mM Tris, 500mM NaCl, 10% Glycerol (pH 7.0).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Mouse IL-5 R alpha, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-5, His-Avi Tag with an affinity constant of 0.511 uM as determined in SPR assay (Biacore T200).
	Biotinylated Mouse IL-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.