Biotinylated Mouse IL-5 Protein (IL5-MM401B)

  • Express System: Expi293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: The affinity constant of 0.511 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Met21-Gly133

Catalog: IL5-MM401B

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Product Description Recombinant Biotinylated Mouse IL-5 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.It contains Met21-Gly133.
Product Category Recombinant Protein
Target IL-5
Synonyms Interleukin-5; IL-5; T-cell replacing factor; TRF; EDF
Gene/Protein ID P04401
Species Mouse
Exact Sequence Met21-Gly133
Molecular Weight The protein has a predicted MW of 16.0 kDa. Due to glycosylation, the protein migrates to 16 kDa and 20-35 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 0.511 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22μm filtered solution in 20 mM Tris, 500 mM NaCl, 10% glycerol (pH 7.0). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
Mouse IL-5 R alpha, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-5, His-Avi Tag with an affinity constant of 0.511 μM as determined in SPR assay (Biacore T200).
The purity of Biotinylated Mouse IL-5 is greater than 95% as determined by SEC-HPLC.
Biotinylated Mouse IL-5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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