Biotinylated Mouse IL-5 Protein (IL5-MM401B)

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Biotinylated: yes
Activity: The affinity constant of 0.511 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Met21-Gly133

Catalog: IL5-MM401B

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Product Description	Recombinant Biotinylated Mouse IL-5 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Met21-Gly133.
Product Background	IL-5 is an important cytokine for priming and survival of mature eosinophils and for proliferation and maturation of their progenitors. IL-5(Rα) targeting will be increasingly used in diseases where eosinophils are the key immune effector cells such as eosinophilic asthma (EA), hypereosinophilic syndrome (HES), eosinophilic esophagitis (EE), and eosinophilic granulomatosis with polyangiitis (EGPA).
Product Category	Recombinant Protein

Target	IL-5
Synonyms	Interleukin-5; IL-5; T-cell replacing factor; TRF; EDF
Gene/Protein ID	P04401
Species	Mouse
Exact Sequence	Met21-Gly133
Molecular Weight	The protein has a predicted MW of 16.0 kDa. Due to glycosylation, the protein migrates to 16 kDa and 20-35 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.511 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22um filtered solution in 20mM Tris, 500mM NaCl, 10% Glycerol (pH 7.0).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Mouse IL-5 R alpha, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-5, His-Avi Tag with an affinity constant of 0.511 uM as determined in SPR assay (Biacore T200).
	The purity of Biotinylated Mouse IL-5 is greater than 95% as determined by SEC-HPLC.
	Biotinylated Mouse IL-5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.