• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Amino Acid Range: Tyr24-His1088 (ITGAL) acidic tail & Gln24-Asn702 (ITGB2) basic tail
Catalog #ITG-MM4L1B
Price
Regular price $66000
Regular price Sale price $66000
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Mouse Integrin alpha L beta 2 (ITGAL&ITGB2) Heterodimer Protein (ITG-MM4L1B) Summary

Product Description

 Recombinant Biotinylated Mouse Integrin alpha L beta 2 (ITGAL&ITGB2) Heterodimer Protein is expressed as a heterodimer of ITGAL and ITGB2 by co-expression in HEK293. Subunit ITGAL is fused with a His tag, an Avi tag and an acidic tail at the C-terminus, subunit ITGB2 is fused with a basic tail at the C-terminus without tag.It contains Tyr24-His1088 (ITGAL) acidic tail and Gln24-Asn702 (ITGB2) basic tail.

Product Background

 Integrin αLβ2 is one member of the integrin family and also called leukocyte function-associated antigen-1 (LFA-1). It is widely expressed on T lymphocytes and controls the function and, in particular, the migration of immune cells[1]. Active αLβ2 can bind ICAM­1/CD54, ICAM­2, ICAM3 and JAM­1[2].

Biotinylated Mouse Integrin alpha L beta 2 (ITGAL&ITGB2) Heterodimer Protein (ITG-MM4L1B) Specifications/Details

Protein
 Integrin alpha L beta 2 (ITGAL&ITGB2) Heterodimer
Synonyms
 αLβ2; ITGAL&ITGB2; ITGAL; ITGB2;aLb2; alpha L beta 2
Accession
Species
 Mouse
Biotinylated
 yes
Amino Acid Range
 Tyr24-His1088 (ITGAL) acidic tail & Gln24-Asn702 (ITGB2) basic tail
Molecular Weight
 The protein has a predicted MW of 125.22 kDa (ITGAL) and 79.60 kDa (ITGB2). Due to glycosylation, the protein migrates to 170-200 kDa (ITGAL) and 95-115 kDa (ITGB2) based on Bis-Tris PAGE result.
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Mouse ITGAL&ITGB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Mouse ITGAL&ITGB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse ITGAL&ITGB2 is greater than 90% as determined by SEC-HPLC. The purity of Biotinylated Mouse ITGAL&ITGB2 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...