- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Mouse OX40 Ligand, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse OX40, His Tag with the EC50 of 1.36ug/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Val20-Pro211
Product Description |
Recombinant Biotinylated Mouse OX40/TNFRSF4/CD134 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val20-Pro211. |
Product Background |
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation. |
Product Category |
Recombinant Protein |
Target |
OX40/TNFRSF4/CD134 |
Synonyms |
CD134;OX40; OX40L receptor; TNFRSF4;ACT-135; Ly-70; Txgp1; TXGP1L; |
Accession |
|
Species |
Mouse |
Biotinylated |
yes |
Amino Acid Range |
Val20-Pro211 |
Molecular Weight |
The protein has a predicted MW of 24.2 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse OX40 Ligand, hFc Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Mouse OX40, His Tag with the EC50 of 1.36ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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