Name: Biotinylated Mouse PD-L1/B7-H1 Protein (Primary Amine Labeling) (PDL-MM110B)
Brand: KactusBio
SKU: PDL-MM110B-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated Mouse PD-L1/B7-H1 Protein (Primary Amine Labeling) (PDL-MM110B)

Biotinylated Mouse PD-L1, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse PD-1 , hFc Tag with an affinity constant of 0.31 uM as determined in SPR assay (Biacore T200).

Biotinylated Mouse PD-L1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Mouse PD-L1 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.31 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Phe19-Thr238

Catalog #PDL-MM110B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Mouse PD-L1/B7-H1 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Phe19-Thr238.
Product Background	B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	PD-L1/B7-H1
Synonyms	CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1
Accession	NP_068693
Species	Mouse
Biotinylated	yes
Amino Acid Range	Phe19-Thr238
Molecular Weight	The protein has a predicted MW of 25.9 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 0.31 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Biotinylated Mouse PD-L1, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse PD-1 , hFc Tag with an affinity constant of 0.31 uM as determined in SPR assay (Biacore T200).
	Biotinylated Mouse PD-L1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Mouse PD-L1 is greater than 95% as determined by SEC-HPLC.