Biotinylated Mouse PD-L1/B7-H1 Protein (Primary Amine Labeling) (PDL-MM110B)
Catalog: PDL-MM110B
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| Product Description | Recombinant Biotinylated Mouse PD-L1/B7-H1 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Phe19-Thr238. |
Product Background |
B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. |
Product Category |
Recombinant Protein |
Target |
PD-L1/B7-H1 |
| Synonyms | CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1 |
Gene/Protein ID |
NP_068693 |
Species |
Mouse |
Exact Sequence |
Phe19-Thr238 |
| Molecular Weight | The protein has a predicted MW of 25.9 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result. |
Activity |
The affinity constant of 3.06 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Mouse PD-1, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Mouse PD-L1, His Tag with an affinity constant of 3.06 uM as determined in SPR assay (Biacore T200). |
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The purity of Biotinylated Mouse PD-L1 is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Mouse PD-L1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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