Biotinylated Mouse PD-L1/B7-H1 Protein (Primary Amine Labeling) (PDL-MM110B)
Biotinylated Mouse PD-L1/B7-H1 Protein (Primary Amine Labeling) (PDL-MM110B)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.31 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Phe19-Thr238
Catalog #PDL-MM110B
Regular price $525.00
/
Size

Order now, ships by:

Product Description
Recombinant Biotinylated Mouse PD-L1/B7-H1 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-Terminus.It contains Phe19-Thr238.
Product Background
B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance.
Product Category
Recombinant Protein
Target
PD-L1/B7-H1
Synonyms
CD274; PDL1; PD-L1; PD-L1B7 homolog 1;B7-H; B7H1; B7-H1; PDCD1L1; PDCD1LG1
Accession
Species
Mouse
Biotinylated
yes
Amino Acid Range
Phe19-Thr238
Molecular Weight
The protein has a predicted MW of 25.9 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 0.31 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Mouse PD-L1, His Tag captured on CM5 Chip via anti-his antibody can bind Mouse PD-1 , hFc Tag with an affinity constant of 0.31 uM as determined in SPR assay (Biacore T200).
Biotinylated Mouse PD-L1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse PD-L1 is greater than 95% as determined by SEC-HPLC.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed