Biotinylated Mouse Peptide Ready H-2K(b)&B2M Monomer Protein (MHC-MM50RB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: His24-Pro297(H-2K(b)) & Ile21-Met119(B2M)
Catalog #MHC-MM50RB
Regular price $341.00
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Biotinylated Mouse Peptide Ready H-2K(b)&B2M Monomer Protein (MHC-MM50RB) Summary

Product Description

Recombinant Biotinylated Mouse Peptide Ready H-2K(b)&B2M Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains His24-Pro297 (H-2K(b)) and Ile21-Met119(B2M).

Product Background

H-2K(b)&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching H-2K(b). Peptide ready MHC molecules comprising mouse H-2K(b) alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.

Product Category

Recombinant Protein / Antibody discovery / MHC / Peptide-ready MHCs

Peptide Ready H-2K(b)&B2M Specifications

Protein
Peptide Ready H-2K(b)&B2M
Synonyms
MHC; H-2K(b); Peptide Ready

MHC Form

Monomer

Allele

H-2K(b)
Accession
Species
Mouse
Biotinylated
yes
Amino Acid Range
His24-Pro297(H-2K(b)) & Ile21-Met119(B2M)
Molecular Weight
The protein has a predicted MW of 48.3 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Product Tag
C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in PBS, 100mM L-Arginine (pH 7.4).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Mouse Peptide Ready H-2K(b)&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Mouse Peptide Ready H-2K(b)&B2M Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse Peptide Ready H-2K(b)&B2M Monomer is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Mouse Peptide Ready H-2K(b)&B2M Monomer is greater than 95% as determined by SEC-HPLC.
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