Biotinylated Mouse PLAU/uPA Protein (PLA-MM401B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.19 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly21-Phe433
Catalog #PLA-MM401B
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Product Description
 Recombinant Biotinylated Mouse PLAU/uPA Protein is expressed from HEK293 with His tag and Avi Tag at the C-Terminus.It contains Gly21-Phe433.
Product Background
 Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC.
Product Category
 Recombinant Protein
Target
 PLAU/uPA
Synonyms
 PLAU; Urokinase; ATF; UPA; URK; u-PA; BDPLT5; QPD
Accession
Species
 Mouse
Biotinylated
 yes
Amino Acid Range
 Gly21-Phe433
Molecular Weight
 The protein has a predicted MW of 49.00 kDa. Due to enzyme lysis and glycosylation, the protein migrates to 30-35 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 0.19 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mouse uPAR isoform 1, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse PLAU, His-Avi Tag with an affinity constant of 0.19 uM as determined in SPR assay (Biacore T200).
Biotinylated Mouse PLAU on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Mouse PLAU is greater than 95% as determined by SEC-HPLC.

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