Biotinylated Mouse PVRIG Protein (PVR-MM501B)

Express System: HEK293
Product Tag: C-hFc-Avi
Purity: > 95% as determined by Tris-Bis PAGE
Biotinylated: yes
Activity: Immobilized Mouse Nectin-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse PVRIG, hFc Tag with the EC50 of 1.76ug/ml determined by ELISA. The affinity constant of 2.22 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ser35-Asp165

Catalog: PVR-MM501B

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Product Description	Recombinant Biotinylated Mouse PVRIG Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Ser35-Asp165.
Product Background	Murine PVRIG interacted weakly with poliovirus receptor (PVR) but bound poliovirus receptor-like 2 (PVRL2) strongly, making the latter its principal ligand.PVRIG is an inducible checkpoint receptor and that targeting PVRIG-PVRL2 interactions results in increased CD8+ T-cell function and reduced tumor growth.
Product Category	Recombinant Protein

Target	PVRIG
Gene/Protein ID	A0A1B0GS01
Species	Mouse
Exact Sequence	Ser35-Asp165
Molecular Weight	The protein has a predicted MW of 42.7 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Mouse Nectin-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse PVRIG, hFc Tag with the EC50 of 1.76ug/ml determined by ELISA. The affinity constant of 2.22 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Biotinylated Mouse PVRIG, hFc-Avi Tag captured on CM5 Chip via Protein A can bind Mouse Nectin-2, His Tag with an affinity constant of 2.22 uM as determined in SPR assay (Biacore T200).
	Immobilized Mouse Nectin-2, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse PVRIG, hFc Tag with the EC50 of 1.76ug/ml determined by ELISA.
	Biotinylated Mouse PVRIG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.