Product Description |
Recombinant Biotinylated Mouse PVRIG Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Ser35-Asp165. |
Product Background |
Murine PVRIG interacted weakly with poliovirus receptor (PVR) but bound poliovirus receptor-like 2 (PVRL2) strongly, making the latter its principal ligand.PVRIG is an inducible checkpoint receptor and that targeting PVRIG-PVRL2 interactions results in increased CD8+ T-cell function and reduced tumor growth. |
Product Category |
Recombinant Protein |
Target |
PVRIG |
Synonyms |
C7orf15; CD112R; MGC104322; MGC138297; MGC2463; PVRIG; ALS2CR18; ALS2CR9; LPD; PREL-2; PREL2; RalGDS/AF-6; RMO1 |
Accession |
|
Species |
Mouse |
Biotinylated |
yes |
Amino Acid Range |
Ser35-Asp165 |
Molecular Weight |
The protein has a predicted MW of 42.7 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Mouse Nectin-2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse PVRIG, hFc Tag with the EC50 of 5.04ug/ml determined by ELISA (QC Test). The affinity constant of 2.22 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Biotinylated Mouse PVRIG, hFc-Avi Tag captured on CM5 Chip via Protein A can bind Mouse Nectin-2, His Tag with an affinity constant of 2.22 uM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Mouse Nectin-2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Mouse PVRIG, hFc Tag with the EC50 of 5.04ug/ml determined by ELISA (QC Test). |
|
|
Biotinylated Mouse PVRIG on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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