Biotinylated Mouse uPAR/PLAUR Protein (PAR-MM401B)
Catalog: PAR-MM401B
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| Product Description | Recombinant Biotinylated Mouse uPAR/PLAUR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu24-Gly298. |
Product Background |
The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR. |
Product Category |
Recombinant Protein |
Target |
uPAR/PLAUR |
Gene/Protein ID |
P35456-1 |
Species |
Mouse |
Exact Sequence |
Leu24-Gly298 |
| Molecular Weight | The protein has a predicted MW of 32.92 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result. |
Activity |
The affinity constant of 6.35 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Biotinylated Mouse uPAR, His Tag immobilized on CM5 Chip can bind Human PLAU, His Tag with an affinity constant of 6.35 nM as determined in SPR assay (Biacore T200). |
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The purity of Biotinylated Mouse uPAR is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Mouse uPAR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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