Product Description |
Recombinant Biotinylated Mouse uPAR/PLAUR Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu24-Gly298. |
Product Background |
The receptor (u-PAR) for urokinase plasminogen activator (u-PA) is a three-domain protein, GPI-anchored to the cell surface, which focuses the enzymatic activity of u-PA, and allows the cell surface activation of plasminogen.Regulation of the activity of u-PA is also mediated by u-PAR. |
Product Category |
Recombinant Protein |
Target |
uPAR/PLAUR |
Accession |
|
Species |
Mouse |
Biotinylated |
yes |
Amino Acid Range |
Leu24-Gly298 |
Molecular Weight |
The protein has a predicted MW of 32.92 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 418.50 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
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Human PLAU, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse uPAR, His Tag with an affinity constant of 418.50 nM as determined in SPR assay (Biacore T200). |
|
|
Biotinylated Mouse uPAR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Mouse uPAR is greater than 95% as determined by SEC-HPLC. |
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