Size-exclusion chromatography (SEC) analysis of biotinylated recombinant rat FGL2 protein, showing a single peak at ~8.87 minutes, indicating high purity and monodispersity.
Biotinylated Rat FGL2 Protein (FGL-RM612B)
  • Express System: HEK293
  • Product Tag: N-His-Avi,N-Flag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Val193-Pro427
Catalog #FGL-RM612B
Regular price $577.50
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Biotinylated Rat FGL2 Protein (FGL-RM612B) Summary

Product Description

Recombinant Biotinylated Rat FGL2 Protein is expressed from HEK293 with His tag and Avi tag and Flag tag at the N-Terminus.It contains Val193-Pro427.

Product Background

Fibrinogen-like protein 2 (FGL2) is a member of the fibrinogen-like protein family and possesses important regulatory functions in both innate and adaptive immune responses. FGL2 is overexpressed in glioma, and its expression level is negatively associated with the prognosis of glioma patients.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Rat FGL2 Protein (FGL-RM612B) Specifications/Details

Protein
FGL2
Synonyms
Fibroleukin; pT49; FGL2; fibrinogen-like 2;
Accession
Species
Rat
Biotinylated
yes
Amino Acid Range
Val193-Pro427
Molecular Weight
The protein has a predicted MW of 31.31 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
Product Tag
N-His-Avi,N-Flag
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Biotinylated Rat FGL2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Rat FGL2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Rat FGL2 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Rat FGL2 is greater than 95% as determined by SEC-HPLC.
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