- Express System: E.coli
- Product Tag: N-His
- Purity: > 95% as determined by Bis-Tris PAGE
- Activity: Immobilized Human GFRAL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Rat GDF15, His Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Ser189-Ala303
Product Description |
Recombinant Biotinylated Rat GDF15 Protein (Primary Amine Labeling) is expressed from E.coli with His tag at the N-terminus.It contains Ser189-Ala303. |
Product Background |
Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15. |
Product Category |
Recombinant Protein |
Target |
GDF15 |
Accession |
|
Species |
Rat |
Biotinylated |
yes |
Amino Acid Range |
Ser189-Ala303 |
Molecular Weight |
The protein has a predicted MW of 13.68 kDa. The protein migrates to 14-17 kDa under reduced (R) condition and 25-30 kDa under Non reducing (N) condition based on Bis-Tris PAGE result. |
Activity |
Immobilized Human GFRAL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Rat GDF15, His Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in 50mM HAc. |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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