Biotinylated Rat GDF15 Protein (Primary Amine Labeling) (GDF-RE115B)
  • Express System: E.coli
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human GFRAL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Rat GDF15, His Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser189-Ala303
Catalog #GDF-RE115B
Regular price $600.00
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Product Description
Recombinant Biotinylated Rat GDF15 Protein (Primary Amine Labeling) is expressed from E.coli with His tag at the N-terminus.It contains Ser189-Ala303.
Product Background
Growth Differentiation Factor 15 (GDF15), also known as NSAID activated gene-1 (NAG-1), is associated with a large number of biological processes and diseases, including cancer and obesity. GDF15 is synthesized as pro-GDF15, is dimerized, and is cleaved and secreted into the circulation as a mature dimer GDF15.
Product Category
Recombinant Protein
Target
GDF15
Accession
Species
Rat
Biotinylated
yes
Amino Acid Range
Ser189-Ala303
Molecular Weight
The protein has a predicted MW of 13.68 kDa. The protein migrates to 14-17 kDa under reduced (R) condition and 25-30 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Activity
Immobilized Human GFRAL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Rat GDF15, His Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
Product Tag
N-His
Express System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22 um filtered solution in 50mM HAc.
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human GFRAL, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Rat GDF15, His Tag with the EC50 of 4.4ng/ml determined by ELISA.
Biotinylated Rat GDF15 on Bis-Tris PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.
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