• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Rhesus macaque CDCP-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Phe30-Thr667
Catalog #CDC-CM401B-UL
Price
Regular price $63525
Regular price Sale price $63525
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Rhesus macaque CDCP1 Protein, Ultra Low Endotoxin (CDC-CM401B-UL) Summary

Product Description

 Recombinant Biotinylated Rhesus macaque CDCP1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe30-Thr667.

Product Background

 Tumor metastasis depends on the dynamic regulation of cell adhesion through β1-integrin. The Cub-Domain Containing Protein-1, CDCP1, is a transmembrane glycoprotein which regulates cell adhesion. Overexpression and loss of CDCP1 have been observed in the same cancer types to promote metastatic progression.

Biotinylated Rhesus macaque CDCP1 Protein, Ultra Low Endotoxin (CDC-CM401B-UL) Specifications/Details

Protein
 CDCP1
Synonyms
 CDCP1; CD318; SIMA135; TRASK; UNQ2486; PRO5773
Accession
Species
 Rhesus macaque
Biotinylated
 yes
Amino Acid Range
 Phe30-Thr667
Molecular Weight
 The protein has a predicted MW of 75 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Rhesus macaque CDCP-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA (QC Test).
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Rhesus macaque CDCP-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Rhesus macaque CDCP-1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-CDCP1 Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA (QC Test).
Biotinylated Rhesus macaque CDCP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Rhesus macaque CDCP1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Rhesus macaque CDCP1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Rhesus macaque CDCP1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...