Biotinylated SARS-COV-2 Spike RBD Protein (COV-VM4BDB)
Biotinylated SARS-COV-2 Spike RBD Protein (COV-VM4BDB)
Biotinylated SARS-COV-2 Spike RBD Protein (COV-VM4BDB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated SARS-COV-2 Spike RBD, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Arg319-Asn532
Catalog #COV-VM4BDB
Regular price $577.50
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Biotinylated SARS-COV-2 Spike RBD Protein (COV-VM4BDB) Summary

Product Description

Recombinant Biotinylated SARS-COV-2 Spike RBD Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg319-Asn532.

Product Background

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated SARS-COV-2 Spike RBD Protein (COV-VM4BDB) Specifications/Details

Protein
Spike RBD
Synonyms
S protein RBD; S glycoprotein RBD; Spike protein RBD
Accession
Species
SARS-COV-2
Biotinylated
yes
Amino Acid Range
Arg319-Asn532
Molecular Weight
The protein has a predicted MW of 27 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated SARS-COV-2 Spike RBD, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated SARS-COV-2 Spike RBD, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA. Immobilized Biotinylated SARS-COV-2 Spike RBD, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 7.7ng/ml determined by ELISA.
Biotinylated SARS-COV-2 Spike RBD on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated SARS-COV-2 Spike RBD on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated SARS-COV-2 Spike RBD is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated SARS-COV-2 Spike RBD is greater than 95% as determined by SEC-HPLC.
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