Name: Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein (COV-VM4FOB)
Brand: KactusBio
SKU: COV-VM4FOB-100UG
Price: 600.0 USD
Availability: InStock

Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein (COV-VM4FOB)

Immobilized Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer, His Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA.

Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer, His Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F)

Catalog #COV-VM4FOB

Size

100UG

500UG

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Product Description	Recombinant Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Product Background	The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Product Category	Recombinant Protein / Antibody discovery

Protein	Spike S (Omicron B.1.1.529) Trimer
Synonyms	S protein; Spike glycoprotein; S glycoprotein; COVID-19
Accession	QHO60594.1
Species	SARS-COV-2
Biotinylated	yes
Amino Acid Range	Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F)
Molecular Weight	The protein has a predicted MW of 136.70 kDa. Due to glycosylation, the protein migrates to 140-180 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer, His Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer, His Tag at 2ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 7.2ng/ml determined by ELISA.
	Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.