Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein (COV-VM4FOB)
Catalog: COV-VM4FOB
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Product Description | Recombinant Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. |
Product Category | Recombinant Protein |
Target | Spike S (Omicron B.1.1.529) Trimer |
Synonyms | S protein; Spike glycoprotein; S glycoprotein; COVID-19 |
Gene/Protein ID | QHO60594.1 |
Species | SARS-COV-2 |
Exact Sequence | Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F) |
Molecular Weight | The protein has a predicted MW of 136.70 kDa. Due to glycosylation, the protein migrates to 140-180 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human ACE2, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer, His Tag with the EC50 of 0.91ug/ml determined by ELISA. See testing image for detail. |
Product Tag | C-His-Avi |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Liquid |
Shipping | Shipped with blue ice. |
Formulation | Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label. |
Stability And Storage | Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles. |
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