Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein (COV-VM4FOB)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Human ACE2, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer, His Tag with the EC50 of 0.91ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F)

Catalog: COV-VM4FOB

$750.00
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Product Description Recombinant Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F). Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
Product Category Recombinant Protein
Target Spike S (Omicron B.1.1.529) Trimer
Synonyms S protein; Spike glycoprotein; S glycoprotein; COVID-19
Gene/Protein ID QHO60594.1
Species SARS-COV-2
Exact Sequence Val16-Pro1213(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H, N764K, D796Y, N856K, Q954H, N969K, L981F)
Molecular Weight The protein has a predicted MW of 136.70 kDa. Due to glycosylation, the protein migrates to 140-180 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human ACE2, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer, His Tag with the EC50 of 0.91ug/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
Immobilized Human ACE2, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer, His Tag with the EC50 of 0.91ug/ml determined by ELISA.
Biotinylated SARS-COV-2 Spike S (Omicron B.1.1.529) Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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