Biotinylated SARS-COV-2 Spike S Trimer Protein (COV-VM4SSB)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated SARS-COV-2 Spike S Trimer, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human ACE2, hFc Tag with the EC50 of 36.5ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Val16-Glu1188

Catalog: COV-VM4SSB

$750.00
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Product Description Recombinant Biotinylated SARS-COV-2 Spike S Trimer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Val16-Glu1188.
Product Category Recombinant Protein
Target Spike S Trimer
Synonyms S protein; Spike glycoprotein; S glycoprotein; COVID-19
Gene/Protein ID QHD43416.1
Species SARS-COV-2
Exact Sequence Val16-Glu1188
Molecular Weight The protein has a predicted MW of 136.6 kDa. Due to glycosylation, the protein migrates to 175-230 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated SARS-COV-2 Spike S Trimer, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human ACE2, hFc Tag with the EC50 of 36.5ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated SARS-COV-2 Spike S Trimer, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human ACE2, hFc Tag with the EC50 of 36.5ng/ml determined by ELISA.
The purity of Biotinylated SARS-COV-2 Spike S Trimer Protein is greater than 95% as determined by SEC-HPLC.
Biotinylated SARS-COV-2 Spike S Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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