Biotinylated SARS-COV-2 Spike S Trimer Protein (COV-VM4SSB)
Biotinylated SARS-COV-2 Spike S Trimer Protein (COV-VM4SSB)
Biotinylated SARS-COV-2 Spike S Trimer Protein (COV-VM4SSB)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Biotinylated SARS-COV-2 Spike S (Trimer) , His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Val16-Glu1188
Catalog #COV-VM4SSB
Regular price $600.00
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Product Description

Recombinant Biotinylated SARS-COV-2 Spike S Trimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val16-Glu1188.

Product Background

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Product Category

Recombinant Protein / Antibody discovery

Protein

Spike S Trimer

Synonyms

S protein; Spike glycoprotein; S glycoprotein; COVID-19

Accession

Species

SARS-COV-2

Biotinylated

yes

Amino Acid Range

Val16-Glu1188

Molecular Weight

The protein has a predicted MW of 136.6 kDa. Due to glycosylation, the protein migrates to 140-170 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated SARS-COV-2 Spike S (Trimer) , His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated SARS-COV-2 Spike S (Trimer) , His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. Immobilized Biotinylated SARS-COV-2 Spike S (Trimer) , His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.
Biotinylated SARS-COV-2 Spike S Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated SARS-COV-2 Spike S Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

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