Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) Protein (COV-VM4SOB)
Catalog: COV-VM4SOB
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Product Description | Recombinant Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gln14-Arg683(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H). |
Product Category | Recombinant Protein |
Target | Spike S1 (Omicron B.1.1.529) |
Synonyms | S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1 |
Gene/Protein ID | QHO60594.1 |
Species | SARS-COV-2 |
Exact Sequence | Gln14-Arg683(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H) |
Molecular Weight | The protein has a predicted MW of 77.94 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Biotinylated Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) , His Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.25μg/ml determined by ELISA. See testing image for detail. |
Product Tag | C-His-Avi |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% saccharose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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