Biotinylated SARS-COV-2 Spike S1 Protein (COV-VM4S1B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated SARS-COV-2 Spike S1, Hisi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 25.5ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln14-Arg683

Catalog: COV-VM4S1B

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Product Description Recombinant Biotinylated SARS-COV-2 Spike S1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln14-Arg683.
Product Category Recombinant Protein
Target Spike S1
Synonyms S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
Gene/Protein ID QHD43416.1
Species SARS-COV-2
Exact Sequence Gln14-Arg683
Molecular Weight The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated SARS-COV-2 Spike S1, Hisi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 25.5ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated SARS-COV-2 Spike S1, Hisi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 25.5ng/ml determined by ELISA.
The purity of Biotinylated SARS-COV-2 Spike S1 is greater than 95% as determined by SEC-HPLC.
Biotinylated SARS-COV-2 Spike S1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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