Product Description |
Recombinant Biotinylated SARS-COV-2 Spike S1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln14-Arg683. |
Product Background |
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. |
Product Category |
Recombinant Protein |
Target |
Spike S1 |
Synonyms |
S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1 |
Accession |
|
Species |
SARS-COV-2 |
Biotinylated |
yes |
Amino Acid Range |
Gln14-Arg683 |
Molecular Weight |
The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated SARS-COV-2 Spike S1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Biotinylated SARS-COV-2 Spike S1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA. |
|
|
Biotinylated SARS-COV-2 Spike S1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Biotinylated SARS-COV-2 Spike S1 is greater than 95% as determined by SEC-HPLC. |
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