Biotinylated SARS-COV-2 Spike S1 Protein (COV-VM4S1B)
Biotinylated SARS-COV-2 Spike S1 Protein (COV-VM4S1B)
Biotinylated SARS-COV-2 Spike S1 Protein (COV-VM4S1B)
Biotinylated SARS-COV-2 Spike S1 Protein (COV-VM4S1B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated SARS-COV-2 Spike S1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln14-Arg683
Catalog #COV-VM4S1B
Regular price $525.00
/
Size

Order now, ships by:

Product Description

Recombinant Biotinylated SARS-COV-2 Spike S1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln14-Arg683.

Product Background

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Product Category

Recombinant Protein / Antibody discovery

Protein

Spike S1

Synonyms

S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1

Accession

Species

SARS-COV-2

Biotinylated

yes

Amino Acid Range

Gln14-Arg683

Molecular Weight

The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated SARS-COV-2 Spike S1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated SARS-COV-2 Spike S1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA. Immobilized Biotinylated SARS-COV-2 Spike S1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA.
Biotinylated SARS-COV-2 Spike S1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated SARS-COV-2 Spike S1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated SARS-COV-2 Spike S1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated SARS-COV-2 Spike S1 is greater than 95% as determined by SEC-HPLC.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed