Biotinylated SARS-COV-2 Spike S1 Protein (COV-VM5S1B)
Biotinylated SARS-COV-2 Spike S1 Protein (COV-VM5S1B)
Biotinylated SARS-COV-2 Spike S1 Protein (COV-VM5S1B)
  • Express System: HEK293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated SARS-COV-2 Spike S1, hFc Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, His Tag with the EC50 of 0.09ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln14-Arg683
Catalog #COV-VM5S1B
Regular price $577.50
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Product Description

Recombinant Biotinylated SARS-COV-2 Spike S1 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Gln14-Arg683.

Product Background

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

Spike S1

Synonyms

S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1

Accession

Species

SARS-COV-2

Biotinylated

yes

Amino Acid Range

Gln14-Arg683

Molecular Weight

The protein has a predicted MW of 102.6 kDa. Due to glycosylation, the protein migrates to 130-140 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated SARS-COV-2 Spike S1, hFc Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, His Tag with the EC50 of 0.09ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 24 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated SARS-COV-2 Spike S1, hFc Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, His Tag with the EC50 of 0.09ug/ml determined by ELISA. Immobilized Biotinylated SARS-COV-2 Spike S1, hFc Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human ACE2, His Tag with the EC50 of 0.09ug/ml determined by ELISA.
Biotinylated SARS-COV-2 Spike S1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated SARS-COV-2 Spike S1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated SARS-COV-2 Spike S1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated SARS-COV-2 Spike S1 is greater than 95% as determined by SEC-HPLC.

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