Biotinylated SARS-COV-2 Spike S1 Protein (COV-VM5S1B)

  • Express System: Expi293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated SARS-COV-2 Spike S1, hFc Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human ACE2, His Tag with the EC50 of 0.22μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln14-Arg683

Catalog: COV-VM5S1B

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Product Description Recombinant Biotinylated SARS-COV-2 Spike S1 Protein is expressed from Expi293 with hFc tag and Avi tag at the C-terminal.It contains Gln14-Arg683.
Product Category Recombinant Protein
Target Spike S1
Synonyms S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
Gene/Protein ID QHD43416.1
Species SARS-COV-2
Exact Sequence Gln14-Arg683
Molecular Weight The protein has a predicted MW of 102.6 kDa. Due to glycosylation, the protein migrates to 130-140 kDa based on Tris-Bis PAGE result.
Activity Immobilized Biotinylated SARS-COV-2 Spike S1, hFc Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human ACE2, His Tag with the EC50 of 0.22μg/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated SARS-COV-2 Spike S1, hFc Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human ACE2, His Tag with the EC50 of 0.22μg/ml determined by ELISA.
The purity of Biotinylated SARS-COV-2 Spike S1 is greater than 95% as determined by SEC-HPLC.
Biotinylated SARS-COV-2 Spike S1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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