Name: Biotinylated SARS Spike RBD Protein (SAR-VM4BDB)
Brand: KactusBio
SKU: SAR-VM4BDB-100UG
Price: 525.0 USD
Availability: InStock

Biotinylated SARS Spike RBD Protein (SAR-VM4BDB)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human ACE2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated SARS Spike RBD, His Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Arg306-Phe527

Catalog #SAR-VM4BDB

Size

100UG

500UG

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Product Description	Recombinant Biotinylated SARS RBD Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg306-Phe527.
Product Background	The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Product Category	Recombinant Protein

Target	Spike RBD
Synonyms	S protein RBD; S glycoprotein RBD; Spike protein RBD
Accession	P59594
Species	SARS
Biotinylated	yes
Amino Acid Range	Arg306-Phe527
Molecular Weight	The protein has a predicted MW of 27.9 kDa. Due to glycosylation, the protein migrates to 36-46 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human ACE2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated SARS Spike RBD, His Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human ACE2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated SARS Spike RBD, His Tag with the EC50 of 0.17ug/ml determined by ELISA.
	Biotinylated SARS RBD on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated SARS RBD is greater than 95% as determined by SEC-HPLC.

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