Biotinylated SARS Spike RBD Protein (SAR-VM4BDB)
Catalog: SAR-VM4BDB
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| Product Description | Recombinant Biotinylated SARS RBD Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg306-Phe527. |
| Product Category | Recombinant Protein |
| Target | Spike RBD |
| Synonyms | S protein RBD; S glycoprotein RBD; Spike protein RBD |
| Gene/Protein ID | P59594 |
| Species | SARS |
| Exact Sequence | Arg306-Phe527 |
| Molecular Weight | The protein has a predicted MW of 27.9 kDa. Due to glycosylation, the protein migrates to 36-46 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human ACE2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated SARS Spike RBD, His Tag with the EC50 of 0.19ug/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human ACE2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated SARS Spike RBD, His Tag with the EC50 of 0.19ug/ml determined by ELISA. |
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The purity of Biotinylated SARS RBD is greater than 95% as determined by SEC-HPLC. |
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Biotinylated SARS RBD on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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