Biotinylated SARS Spike RBD Protein (SAR-VM4BDB)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Human ACE2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated SARS Spike RBD, His Tag with the EC50 of 0.19ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Arg306-Phe527

Catalog: SAR-VM4BDB

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Product Description Recombinant Biotinylated SARS RBD Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg306-Phe527.
Product Category Recombinant Protein
Target Spike RBD
Synonyms S protein RBD; S glycoprotein RBD; Spike protein RBD
Gene/Protein ID P59594
Species SARS
Exact Sequence Arg306-Phe527
Molecular Weight The protein has a predicted MW of 27.9 kDa. Due to glycosylation, the protein migrates to 36-46 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human ACE2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated SARS Spike RBD, His Tag with the EC50 of 0.19ug/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human ACE2, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated SARS Spike RBD, His Tag with the EC50 of 0.19ug/ml determined by ELISA.
The purity of Biotinylated SARS RBD is greater than 95% as determined by SEC-HPLC.
Biotinylated SARS RBD on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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