Canine CD28 Protein (CD8-CM128) Summary
Product Description |
Recombinant Canine CD28 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr18-Pro153. |
Product Background |
CD28 is the receptor for CD80 (B7-1) and CD86 (B7-2) proteins.When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Canine CD28 Protein (CD8-CM128) Specifications/Details
Protein |
CD28 |
Synonyms |
CD28 antigen (Tp44); CD28 molecule; CD28; MGC138290 |
Accession |
|
Species |
Canine |
Biotinylated |
no |
Amino Acid Range |
Thr18-Pro153 |
Molecular Weight |
The protein has a predicted MW of 16.7 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Canine CD28 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Canine CD28 is greater than 95% as determined by SEC-HPLC. |
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